Therapeutic effect of human lysozyme and bovine interferon-γ expression vectors on diary cow mastitis
YIN Zhaohua;ZHANG Yihui;ZHANG Yu;SUN Huaichang;CHEN Yang;ZHANG Xinyu;XIA Xiaoli;Experimental Dairy Farm,Yangzhou University;College of Veterinary Medicine,Yangzhou University;
This study was to compare the therapeutic effect of human lysozyme( LYZ) and/or bovine interferon-γ( BoIFN-γ) expression vector( s) with antibiotics on dairy cow mastitis. BoIFN-γ c DNA was sub-cloned into the eukaryotic expression vector pcDNAK and BoIFN-γ expression in NIH 3 T3 cells was detected by immunofluorescence after transfection with the recombinant vector p CDNAK-BoIFN-γ.Through quarter base punctuation,each quarter of dairy cows with mastitis was injected two times at one-day intervals with 400μg pcDNAKLYZ,pcDNAK-BoINF-γ,pcDNAKLYZ plus pcDNAK-BoINF-γ or 100 U ampicillin plus 25μg streptomycin. For clinical and subclinical mastitis,the pcDNAKLYZ-treated group showed therapeutic efficacies of 80% and 79%,respectively. The pcDNAK-BoINF-γ-treated group showed therapeutic efficacies of 50% and 67%,respectively. The pcDNAKLYZ plus pcDNAK-BoINF-γ-treated group showed therapeutic efficacies of 100% and 84%,respectively. The antibiotics-treated group showed therapeutic efficacies of 50% and 60%,respectively,on the basis of clinical observation and/or somatic cell and total bacterial counting at day 10 after injection. These data suggested that co-injection of human LYZ and BoIFN-γ expression vectors had a higher therapeutic effect than single vector or antibiotics injection.
【CateGory Index】： S858.23