Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Animal Husbandry & Veterinary Medicine》 2018-08
Add to Favorite Get Latest Update

Effect of autophagy on apoptosis induced by zearalenone in TM4 cells

SI Mengxue;WANG Bingjie;FENG Nannan;ZOU Hui;GU Jianhong;YUAN Yan;LIU Xuezhong;LIU Zongping;BIAN Jianchun;College of Veterinary Medicine,Yangzhou University;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses;  
In order to investigate the role of autophagy in the apoptosis of TM4 cells induced by zearalenone( ZEA),TM4 cells were used as experiment model. After exposing TM4 cells to different concentrations of ZEA( 0,5,10 and 20 μmol/L) for 24 h,the flow cytometry was used to detect the apopotic rate,and western blot was used to detect expression of apoptosis-related proteins. The changes in the apoptosis rate,apoptosis-related protein and autophagy-related protein LC-3 in TM4 cells were detected by 20 μmol/L ZEA combined with autophagy chloroquine( CQ) or Rapamycin( Rap). The results showed that,compared with the control group,the ratio of Bax/Bcl-2 and the expression of cleaved-caspase-3 and cleaved-caspase-9 were decreased and the expression of autophagy marker protein LC-3 was increased significantly( P0. 05 or P0. 01). The apoptosis rate increased significantly after the ZEA and CQ,compared with that after the ZEA treatment. The ratio of Bax/Bcl-2,cleaved-caspase-3 and cleaved-caspase-9 and LC-3 protein expressions decreased significantly( P 0. 05 or P0. 01). The apoptosis rate significantly decreased after the ZEA and Rap,compared with that after the ZEA treatment. The ratio of Bax/Bcl-2,cleaved-caspase-3 and cleaved-caspase-9 and LC-3 protein expressions increased significantly( P0. 05 or P0. 01). The apoptotic rate was significantly correlated with the level of autophagy during the apoptosis of TM4 induced by ZEA,and the apoptosis rate of TM4 induced by ZEA was significantly increased. The apoptosis rate of TM4 cells induced by ZEA was significantly decreased. In conclusion: autophagy delayed the apoptosis of TM4 cells and played a protective role in the toxicity of ZEA on TM4 cells.
【Fund】: 国家重点研发计划支持项目(2016YFD0501208);; 江苏高校优势学科建设工程资助项目(PAPD)
【CateGory Index】: S859.8
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved