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《Journal of Southwest University for Nationalities(Natural Science Edition)》 2009-02
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Genetic diversity of gliadin in wild germplasm of Elymus nutans Griseb

MIAO Jia-min1, CHEN Zhi-hua1, 2, ZHONG Jin-cheng1, CHEN Shi-yong2, MA Xiao2, ZHANG Xin-quan2(1.School of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, P.R.C.;2.Department of Grassland Science, Sichuan Agricultural University, Ya'an 625014, P.R.C.)  
Acid polyacrylamide gel electrophoresis(A-PAGE) is used to detect the gliadin genetic polymorphism among sixty-four wild accessions of Elymus nutans Griseb.collected from five provinces and regions in China(Qinghai, Tibet, Sichuan, Gansu and Xinjiang).The results are obtained:(1)A total of 42 bands are detected in all accessions, 90.48% are polymorphic bands.The Nei's genetic similarity coefficient of the tested accessions ranges from 0.320 to 1.000, and the average Nei's coefficient is 0.631.These results suggested that there is rich genetic polymorphism among the tested wild resources of Elymus nutans Griseb.(2) 64 accessions can be clustered into six groups at GS= 0.59 level on dendrogram.The principal coordinates analysis(PCoA) reflected almost the same relationships among the studied materials as shown in cluster analysis.Moreover, the accessions from the same origin are frequently clustered into one group.The findings imply a correlation among gliadin patterns, geographical and ecological environment.
【Fund】: 国家重点基础研究发展计划973项目资助(2007CB108907)
【CateGory Index】: S543.9
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