Assessment of genetic diversity of Elymus nutans in northwest plateau of Sichuan province using ISSR markers
ZHENG Jing-hong;CHEN Shi-yong;CHEN Zhi-hua;LI Shi-dan;ZHONG Jin-cheng;Institute of Qinghai-Tibet Plateau, Southwest University for Nationalities;School of Life Science and Technology, Southwest University for Nationalities;
The genetic diversity of 25 Elymus nutans wild accessions from northwest plateau of Sichuan province was investigated using 10 ISSR primers. A total of 120 were detected, of which a total of 85 bands were polymorphic. The amplified bands for each primer were ranged from 9 to 17, in an average of 12. Likewise, the polymorphic bands for each primer varied from 3-16 bands with a mean of 8.5 bands. The genetic similarity(GS) values based on ISSR data ranged from 0.703-0.974 with a mean of 0.828, indicating a rich genetic diversity. The cluster analysis(UPGMA) and principle component analysis(PCA) based on ISSR data showed that all 25 Elymus nutans could be divided into three groups, which showed some correlation with the geographical origins of E. nutans.