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《Journal of Southwest Agricultural University》 2004-03
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CONSTRUCTION OF THE EXPRESSION VECTORS FOR ARTIFICIAL MALE STERILITY GENE AND FERTILITY RESTORING GENE

SONG Hong-yuan~1, DING Jian-gang~1, CAO Bi-hao~2, LEI Jian-jun~2 ,SONG Ming~1 (1. College of Horticulture and landscape, Southwest Agricultural University, Chongqing 400716, China; 2. Department of Horticulture, South China Agricultural University, Guangzhou,Guangdong, 510642, China)  
The male sterility gene expression vector pCABARTABn and the fertility- restoring gene expression vector pBINPLUSCre were constructed by combining the features of the Cre/loxp site-specific recombination system and F1 hybrid breeding. The sterile gene expression box in pTTABn vector, which contained the TA 29 promotor, barnase gene and nos terminator, was cut down and then inserted in between the 2 directly oriented loxp sites of pGloxp. The final expression vector pCABARTABn was obtained by inserting the sterile gene expression box with the two directly oriented loxp sites into the plasmid pCAMBar, which contained the Bar gene. The Cre gene was cloned from lysogen BM 25.8 with the PCR procedure. The Cre gene was then inserted in between the CaMV 35 S-35 S double promoter and nos terminator of PBI 525 plasmid after sequencing. The expression box was cut down and inserted into plasmid pBINPLUS to obtain the final fertility-restoring gene expression vector pBINPLUSCre.
【Fund】: 国家自然科学基金(30200185);; 重庆市应用基础项目(渝科计字200217)资助
【CateGory Index】: S336
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