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《Journal of Southwest University(Natural Science Edition)》 2007-02
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Sequence Analysis of mtDNA NADH-Ubiquinone Oxidoreductase Chain 1 Gene (ND1) of the Asian Black Bear Sichuan Subspecies (Ursus thibetanus mupinensis)

HOU Wan-ru, CHEN Yu, WU Xia,PENG Zheng-song, ZHOU Cai-quan, YANG JunCollege of Life Science, China West Normal University, Nanchong Sichuan 637002, China  
Primers were designed based on the NADH-ubiquinone oxidoreductase chain 1 gene DNA sequences (ND1) of several species. The total DNA extracted from the hair follicle tissue of the Asian black bear Sichuan subspecies (Ursus thibetanus ssp.mupinensis) was amplified by PCR to obtain the ND1 gene sequence. The softwares GenScan, Blast 2 1, ORF finder and Clustal W were utilized in DNA sequence prediction, similarity comparison, ORF search and multi-sequence alignment, respectively, and the software PredictProtein was used in protein prediction and structure analysis. The result showed that the ND1 gene sequence is 957bp long and contains a 957 nucleotide ORFs (open reading frames) encoding 317 amino acid residues. The amino acid sequence had a pI of 7.83 and a molecular weight of 35.6×103 Da.The DNA sequence of ND1 gene of Asian black bear of Sichuan and the amino acid sequence encoded by it shared relatively high similarity with the DNA sequence and amino acid sequence of other bear species, raccoon (Procyon) and Ailurus fulgens.
【Fund】: 国家自然科学基金资助项目(30570275);; 四川省应用技术项目(2006J13-057);; 四川省教育厅重点科研项目(2004A101);; 西华师范大学科研启动项目(2003);; 四川省重点学科建设项目(SZD0420)
【CateGory Index】: Q343
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