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《Journal of Southwest University(Natural Science Edition)》 2008-06
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Establishment of the Exogenous Gene Expression System Based on the Hairy Roots of Ipomoea batatas(L.) Lam.

HUANG Yuan-she,PENG Mei-fang,LI Ming,LIANG Yuan-yuan,LENG Jin-chuan,ZHANG Qi-tangSchool of Life Science,Southwest University,Chongqing 400715,China  
The disarmed Agrobacterium tumefaciens strain C58C1 harboring the plasmid pRiA4 was engineered by introducing the plant expressing vector pCAMBIA1304+.The bacteria-free well-growing true leaves of 'Yusu 303',a new cultivar of Ipomoea batatas,were infected with the engineered recombined C58C1.Hairy roots were induced from the wounded sites of the leaves at a frequency of 72%.Then,genomic PCR was performed to detect rolC and antihygromycin gene in different monoclonal hairy root lines.Both genes were simultaneously detected in 36% hairy root clones.These results demonstrated that exogenous genes could be expressed in hairy roots of I.batatas at a relatively high frequency by the method in the present studies.It may be a fundamental work for rapid gene functional identification and gene co-transformation into I.batatas and will improve metabolic engineering and molecular breeding of this plant.
【Fund】: 重庆市良种创新工程专项资助项目(10379);; 农业部公益性行业科研专项资助项目(nyhyzx07-012-14)
【CateGory Index】: S531
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