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《Southwest China Journal of Agricultural Sciences》 2010-05
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Transformation of Tolerant-gyphosate 2mG2-epsps Gene by Maize Shoot Tip and Identification of Resistance

YU Gui-rong1,2,DU Wen-ping2,SONG Jun2,DOU Qian2,LIU Yong-sheng1,LU Wei3,XU Li-yuan2(1.College of Life Sciences,Sichuan University,Sichuan Chengdu 610064,China;2.Institute of Biotechnology&Nuclear Techniques,Sichuan Academy of Agricultural Sciences,Sichuan Chengdu 610066,China;3.Biotechnology Research Institute,China Academy of Agricultural Sciences,Beijing 100081,China)  
10 elite maize inbred lines,such as X2211 and 78599,their shoot tip meristematic cell were thansfered via Agrobacterium with plasmid construct that contained 2mG2-epsps gene under vacuum negative pressure.The transgenic plants were co-cultured for 3 days,then planted in flowerpot,selected by glyphosate at 3 leaves and 1 heart-leaf period and tolerant-glyphosate(1 ‰-1.25 ‰)plants were and then,planted out to field.From seed germination to tolerant-plant planted out,the whole time was about 30 days.At the same time,the allele-specific PCR analysis demonstrated that the 2mG2-epsps gene was transformed into the maize genome in some transgenic plants.
【Fund】: 转基因生物新品种培育科技重大专项课题(2009ZX08003-003B转新型高抗草甘膦基因(2mG2-epsps)玉米新组合选育);; 四川省农科院优秀论文基金项目(2009-2010转耐草甘膦兼抗虫玉米研究);; 四川省应用基础项目(07JY029-103-5)
【CateGory Index】: S513
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【Citations】
Chinese Journal Full-text Database 7 Hits
1 Li Xiaoyu;Xu Longzhu; Zhang Genfa(Department of Biolog, Henan Normal University, Xinxiang 153002);HIGH FREQUENCY INDUCTION OF SOMATIC EMBRYOGENESIS IN YOUNG INFLORESCENCES CULTURE OF MAIZE[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1997-03
2 CHEN Li~(1,2),DOU Bing-de~(1,2),LUO Yu-ming~1,CAO Jun-mei~2, ZHANG Yong-xia~1,SUN Rui~1,HE Ye~2,HOU Bei-wei~2(1.Biology Department of Huaiyin Teachers College,Huaian 223300,China)(2.Agronomy College,Xinjiang Agricultural University,Urumqi 830053,China);Establishment of Highly Efficient Regeneration System of Maize Shoot Tip[J];Journal of Huaiyin Teachers College(Natural Science Edition);2006-01
3 He Fuxia1,2 Lang Zhihong2 Lu Wei2 Lin Min2 Zhang Jie3 Huang Dafang2(1College of Life Science,Northeast Agricultural University,Harbin 150030;2Biotechnology Research Institute,Chinese Academy of Agricultural Science,Beijing 100081;3Key Laboratory of Biology of Plant Disease and Insect Pest,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094);The Establishment of Maize Transformation System with a Glyphosate-tolerant 2mG2-epsps Gene as a Selectable Marker[J];Biotechnology Bulletin;2008-05
4 ZHAO Yun-yun~1,ZHOU Xiao-mei~2,WANG Guo-ying~3(1.Department of Biology,Capital Normal University,Beijing 100037,China;2.School of Life Science and Technology,Shanxi University,Taiyuan 030006,China;3.State Key Laboratory for Agrobiotechnology,China Agriculture University,Beijing 100094,China);Study on Tissue Culture and Transformation of Maize[J];Journal of Shanxi University(Natural Science Edition);2006-03
5 SUN Qing-Quan1,2,ZHANG Ying2,RONG Ting-Zhao2,,DONG Shu-Ting1,and ZHANG Chun-Qing1(1 Key Laboratory of Crop Biology,Shandong Agricultural University,Tai'an 271018,Shandong;2 Maize Research Institute,Sichuan Agricultural University, Ya'an 625014,Sichuan,China);Establishment of Transgenic Acceptor and Transformation of barnase Gene by Particle Gun in Maize Inbred Line 18-599(White)[J];Acta Agronomica Sinica;2007-05
6 DU Juan, MU Qiu_hua, JIA Yu_feng, TIAN Li_guo, ZHANG Xin_sheng (Chang Chun University of Agricultural and Animal sciences,Chang Chun 130062 China);Studies on Increasing the Rate of Anther Culture Induction of Maize by the Method of Transform Breeding[J];MAIZE SCIENCES;1999-03
7 DOU Qian1,2,YU Gui-rong2*,DU Wen-ping2,YU Yao-ying2,ZHAO Zhi1,PENG Zheng-song1,XU Li-yuan2 (1.College of Life Science,China West Normal University,Sichuan Nanchong 637002,China;2.Institute of Biotechnology & Nuclear Techniques,Sichuan Academy of Agricultural Sciences,Sichuan Chengdu 610066,China);Establishment of Transgenic Receptor System in Maize Immature Embryos[J];Southwest China Journal of Agricultural Sciences;2010-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Li,CHENG Zhao-xia,YUAN Hong-juan(Life Science Department of Yuncheng University,Yuncheng 044000,China);Study Progress on Callus Inducement of Different Maize Explants[J];Shandong Agricultural Sciences;2009-04
2 ZHENG Ai-zhen (Department of Biology, Shangqiu Teachers College, Shangqiu,Henan 476000);Application and Prospect of Modern Bio-technique in Agriculture[J];Journal of Anhui Agricultural Sciences;2005-07
3 WANG Xiu-qin et al(College of Agronomy,Shandong Agricultural University,Taian,Shandong 271018);Research Progress in Transformation Maize Mediated with Agrobacterium Tumefaciens[J];Journal of Anhui Agricultural Sciences;2006-18
4 WANG Hong-wei et al(Maize Institute,Shenyang Agricultural University,Shenyang,Liaoning 100161);Utilization of Biotechnology in Maize Breeding[J];Journal of Anhui Agricultural Sciences;2007-12
5 SUN Wei et al (School of Life Science,Capital Normal University,Beijing 100097);Progresses in Maize Genetic Transformation Techniques[J];Journal of Anhui Agricultural Sciences;2007-25
6 LIN Gan et al (Department of Chemistry and Bioscience,Xiangfan University,Xiangfan,Hubei 441053);Research Progress on Plant Tissue culture of Maize[J];Journal of Anhui Agricultural Sciences;2007-28
7 WANG Hong-zhen et al(College of Life Science,Jilin Normal University,Siping,Jilin 136000);Study on Callus Induction of Maize Inbred Line 4112 from Immature Embryos with Different Induction Medium[J];Journal of Anhui Agricultural Sciences;2008-30
8 JIANG Su-yun,HU Xiao-rui,CHAO Xiang-rong,YANG Ai-fang(School of Life Science,Shandong University,Ji'nan 250100,China);High efficiency production of multiple shoots and inflorescences induction from the shoot of Lolium multiflorum and L.perenne[J];Acta Pratacultural Science;2005-06
9 GAO Shu-ren1,LI Yan-fang2,DU Juan2( 1. Plant Science College, Heilongjiang August First Land Reclamation University, Daqing Heilongjiang 163319, PRC;2. The Quartermaster University of PLA, Changchun Jilin 130062, PRC );Inducement of callus and plant regeneration of shoot tip in maize[J];Journal of Northeast Agricultural University;2005-03
10 MIN Li,LI Mang-xue,CANG Jing,HE Fu-xia ( Life Science College, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC );Establishment of regeneration system of maize embryo leaf of inbred lines in northeast[J];Journal of Northeast Agricultural University;2005-04
China Proceedings of conference Full-text Database 3 Hits
1 LIU Zhao-jun Institute of Biotechnology Research.Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China; LU Cui-hua WANG Zhen-hua DI Hong Agricultural College,Northeast Agri cultural University,Harbin 150030,China;Study of Maize (Zea mays L.) Transformation by Pollen Tube Pathway[A];[C];2008
2 SHI Hong-xin,LIN Hui,XIANG Ju-ping,LEI Xiao-ming,XU Chun-liu,WU Lei State Key laboratory Breeding Base of Green Chemistry Synthesis Technology,Institute of Chemical Engineering and Material Science,Zhejiang University of Technology,Hangzhou 310032,China Department of Chemistry,Zhejiang University.Hangzhou 310058.China;Synthesis of MeP(OEt)_2-An Intermediate of Glufosinate[A];[C];2005
3 Jiang Wusheng;Yuan Yuxiang;Geng Jianfeng;Zhang Xiaowei;Han Yongping;Gao Muqiang Biological Technologe Institute, Hennan Academy of Agricultural Sciences, Zhengzhou 450002;Study on Maize Haploid Embryonic Induction and Transformation by Particle Bombardments[A];[C];2003
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 FENG Ying-ying,ZHU Yan-ming,ZHANG De-jie,LI Jie,BAI Xi( College of Life Science,Northeast Agricultural University,Harbin Heilongjian g 150030,PRC );Stablishment of maize(Zea mays L.)regeneration system and optimizatio n of its main influencing factors[J];Journal of Northeast Agricultural University;2004-06
2 GAO Shu-ren1,LI Yan-fang2,DU Juan2( 1. Plant Science College, Heilongjiang August First Land Reclamation University, Daqing Heilongjiang 163319, PRC;2. The Quartermaster University of PLA, Changchun Jilin 130062, PRC );Inducement of callus and plant regeneration of shoot tip in maize[J];Journal of Northeast Agricultural University;2005-03
3 Li Xiaoyu;Xu Longzhu; Zhang Genfa(Department of Biolog, Henan Normal University, Xinxiang 153002);HIGH FREQUENCY INDUCTION OF SOMATIC EMBRYOGENESIS IN YOUNG INFLORESCENCES CULTURE OF MAIZE[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1997-03
4 Xiang Fengning;Zhang Juren;Cben Huimin ;and Zheng Xiaojian(Department of Bilogy,Shandong University,Jinan 250100);LONG-TERM CULTURE AND CHROMOSOME VARIATIONS OF EMBRYOGENIC CALLI IN MAIZE[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1994-03
5 FU Feng-ling FENG Zhi-lei QU Bai-yan LI Wan-chen(Key Laboratory of Crop Genetic Resources and Improvement/Ministry of Education,Maize Research Institute,Sichaun Agricultural University,Ya'an,Sichaun 625014);RELATIONSHIPS BETWEEN INDUCTION RATE OF EMBRYONIC CALLUS AND ENDOGENOUS HORMONES CONTENT IN MAIZE[J];Journal of Nuclear Agricultural Sciences;2006-01
6 DU Juan, YU Yun zhou, ZHANG Ling bing, JI Jing, WANG Gang (Research Center for Plant Genetic Engineering ,The Quartermaster University of PLA, Changchun 130062,China);Initiation and Transformation of Callus Ⅱ of Maize Inbred Line[J];Journal of Jilin Agricultural University;2000-04
7 LIU Xi-juan, LIU Yu-hui , WANG Zhi-xing, WANG Xu-jing, ZHANG Yong-qiang (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081,China);Generation of Glyphosate-tolerant Transgenic Tobacco and Cotton by Transformation with a 5-enolpyruvyl-shikimate-3-phosphate Synthase (EPSPS) Gene[J];Journal of Agricultural Biotechnology;2007-06
8 Wang Guoying; Zhang Hong; Xie Youju ;Dai Jingru; Mi Jingjiu(Beijing Agricultural University, Beijing 100094)Li Taiyuan; Tian Yingchuan; Qiao Liya; Mang Keqiang(Institute of Microbiology,CAS,Beijing l00080);Transfer of Bt-toxin Protein Gene into Maize and the Resistance of Transgenic Plants to Corn Borer[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1995-03
9 Xiang Wensheng Zhao Changshan Tao Bo Wang Xiangjing Su Shaoquan(Life Science & Biotechnique Center, Northeast Agriculture University, Harbin 150030) Zhang Wenji(Department of Applied Chemistry, China Agriculture University, Beijing 100094);PRELIMINARY STUDIES ON THE MECHANISM OF GLYPHOSATE TOLERANCE IN BEAN[J];Chinese Journal of Pesticide Science;1999-03
10 Xiang Wensheng 1 Zhang Wenji 2 Wang Xiangjing 1 Qin Zhaohai 2( 1Life Science & Biotechnique Center, Northeast Agricultural University, Harbin 150030) ( 2Department of Applied Chemistry, China Agricultural University, Beijing 100094);Development on Property of EPSP Synthase and Its New Inhibitors[J];Chinese Journal of Pesticide Science;2000-02
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