Tissue Culture and Plant Regeneration of Agave victoriaereginae
LV Ping;WEI Li-jun;JIN Gang;PANG Xin-hua;Guangxi Institute of Subtropical Crops;Guangxi Subtropical Key Laboratory of Advantage Crops Breeding and New Technological Development;
Different Agave victoriaereginae explants and the compounding proportions of different hormones were comparatively studied in tissue culture and thereafter a high-efficient plant-regeneration system by Agave victoriaereginae in vitro culture was established. The medium MS + 6-BA 2. 0 mg·L- 1+ NAA 0. 2 mg·L- 1was the best to multiply shoot from lateral bud of Agave victoriaereginae,with multiplication coefficient of 4. 6. The sequence of callus induction and regeneration of different places in 1eaf was leaf base leaf mid leaf top. Effective callus was only induced from leaf base and they were great explants materials. The induction medium MS + 6-BA 2. 0mg·L- 1+ 1.0 KT mg·L- 1+ NAA 0. 2 mg·L- 1was the best to induce callus from different places in 1eaf and leaf base had 100 % regeneration rate,leaf mid 98 % and leaf top 89 %. And most leaf base could indirectly divide small shoot from callus. Effective callus transferred to the division medium. The results showed that the medium MS + 6-BA 1. 0 mg·L- 1+ KT 1. 5 mg + NAA 0. 2 mg·L- 1produced most differentiation shoots and the growth trend in it was also better than in others. The multiplication effect of shoot was the best in MS + 6-BA 0. 5 mg·L- 1+ KT 1. 0 mg·L- 1+ NAA 0. 2 mg·L- 1,with multiplication rate of 100 % and the average multiplication coefficient of 5.5. In adventitious shoot regenerating,MS + KT 1. 5 mg·L- 1+ NAA0. 5 mg·L- 1was the best formulation,with high rooting frequency and strong root system.