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《Journal of Southwest China Normal University(Natural Science Edition)》 2012-03
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Determination of Cholesterol in Oil and Fat Using Cloud Point Extraction by HPLC-UV

ZHANG Li-xian,ZHOU Guang-ming,JIANG Lan-fang,XU Li Key Laboratory on Luminescence and Real-Time Analysis(Southwest University),Ministry of Education;School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China  
A fast,simple and sensitive sample preparation procedure based on cloud point extraction(CPE) is proposed for the determination of cholesterol in oil and fat using reverse phase high performance liquid chromatography(RP-HPLC) and UV detection.The non-ionic surfactant Triton X-114 was chosen as the extract solvent.The samples were analyzed on a Phenomenex C18 column with ethanol-methanol as the mobile phase using isocratic elution.The flow rate was 0.8 mL·min-1,and UV detection wavelength was set at 206 nm.Optimized conditions for the pretreatment of oil and fat included the concentration of the surfactant Triton X-114,the concentration of sodium chloride,equilibration temperature and equilibration time.A good linear correlation was observed to exist between the concentration of cholesterol in the range of 0.10-150 μg/mL and its peak area(r=0.999 79).The limit of detection(LOD) was 0.03 μg/mL(S/N=3),and the average recovery varied from 86.00% to 93.50%.This new method is fast,easy to operate,sensitive and environment-friendly,and can meet the requirements of determination of cholesterol of oil and fat.
【Fund】: 国家科技重大专项;; 重庆市自然科学基金(CSTC 2007BB5370)
【CateGory Index】: TS227;O657.72
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