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《Journal of Southwest China Normal University(Natural Science Edition)》 2012-03
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Determination of Cholesterol in Oil and Fat Using Cloud Point Extraction by HPLC-UV

ZHANG Li-xian,ZHOU Guang-ming,JIANG Lan-fang,XU Li Key Laboratory on Luminescence and Real-Time Analysis(Southwest University),Ministry of Education;School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China  
A fast,simple and sensitive sample preparation procedure based on cloud point extraction(CPE) is proposed for the determination of cholesterol in oil and fat using reverse phase high performance liquid chromatography(RP-HPLC) and UV detection.The non-ionic surfactant Triton X-114 was chosen as the extract solvent.The samples were analyzed on a Phenomenex C18 column with ethanol-methanol as the mobile phase using isocratic elution.The flow rate was 0.8 mL·min-1,and UV detection wavelength was set at 206 nm.Optimized conditions for the pretreatment of oil and fat included the concentration of the surfactant Triton X-114,the concentration of sodium chloride,equilibration temperature and equilibration time.A good linear correlation was observed to exist between the concentration of cholesterol in the range of 0.10-150 μg/mL and its peak area(r=0.999 79).The limit of detection(LOD) was 0.03 μg/mL(S/N=3),and the average recovery varied from 86.00% to 93.50%.This new method is fast,easy to operate,sensitive and environment-friendly,and can meet the requirements of determination of cholesterol of oil and fat.
【Fund】: 国家科技重大专项;; 重庆市自然科学基金(CSTC 2007BB5370)
【CateGory Index】: TS227;O657.72
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【Citations】
Chinese Journal Full-text Database 7 Hits
1 HUANG Dao Ping CHEN Jian Hong LAO Yan Wen ZHOU Lin MO Jian Guang (Guangxi Research Centre of Analysis,No.32 Xinghulu,Nanning 530022,P.R.China );Determination of Cholesterol in Export Moon Cake by HPLC UV Detector[J];Chinese Journal of Spectroscopy Laboratory;2001-04
2 CHEN Yong-wei,LI Jie,XING Xiu-ying,L Lin (Yingkou Municipal Institute of Supervision and Inspection of Product Quality,Yingkou 115000,China);Rapid Determination of Cholesterol in Oil and Fat[J];Physical Testing and Chemical Analysis(Part B:Chemical Analysis);2009-02
3 GUO Tao,DU Lei-lei,WAN Hui,HE Dong-ping(College of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China);Identification of Hogwash Oil-adulterated Rapeseed Oil through HPLC Detection of Cholesterol Content[J];Food Science;2009-22
4 Zhang Rongzhen, Li Long, Liu Shutao, Chen Ruming and Rao Pingfan (Institute of Biotechnology, Fuzhou University, Fuzhou, 350002);An Improved Method of Cholesterol Determination in Egg Yolk by High Performance Liquid Chromatography[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1998-02
5 LI Zhuo,DONG Wen-bin,LI Na,YANG Jin,WANG Jin-zi,NAN Xue-mei (Shaanxi University of Science and Technology,Xi'an 710021);Cloud-point extraction and its application in pretreatment of food analysis[J];Food Science and Technology;2010-03
6 YUE Zhi -hua,SUN Hui -min and TIAN Song -jiu National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050 GUO Yu-ning Beijing Tiantan Hospital, Chinese Academy of Medical Sciences, Beijing 100050;Study on Simultaneous Quantitative Determination of Cholesterol,Bilirubin and Various Cholic Acids in Human Gallstones by HPLC -ELSD -UV[J];Chinese Journal of Pharmaceutical Analysis;2004-03
7 ZHANG Rui~1,ZU Li-ya~1,FAN Tie~1,ZHAO Ke-peng~2(1.Science Academy of State Grain Administration,100037 Beijing,China;2.Xingtai Quality Supervisory Office,054000 Hebei Xingtai,China);Distinguishing hogwash oil in edible vegetable oil by determination of cholesterol content[J];China Oils and Fats;2006-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WU Zhong-ling1,HE Zhong-qiang1,GUO Mao-zhang1,CHEN Shu-dong1,TANG Wei-qiang2 (1.Guangzhou Quality Supervision and Testing Institute,National Centre for Quality Supervison and Testing of Processed Food,Guangzhou 510110,China;2.School of mechanical & automatic engineering,Guangzhou 510460,China);Determination of Cholesterol in Fat by Solid Phase Extraction—GC/MS[J];Packaging and Food Machinery;2011-06
2 YU Wen-jing1,ZHENG Li1,SHEN Chong-yu2(1.Suzhou Entry & Exit Inspection and Quarantine Bureau,Suzhou 215104,China; 2.Jiangsu Entry & Exit Inspection and Quarantine Bureau,Nanjing 210001,China);Test of Qualitative and Quantitative Method for Cholesterol in Hogwash Oil by LC/MS/MS[J];Journal of Chongqing University of Technology(Natural Science);2011-12
3 LU Qing-ping1 , ZHANG Hong-fu1 , FU Jing2, LIU Fu-zhu2 (1.Animal Science Institute,CAAS,Beijing 100094,China ; 2.Northwest Science Technique University of Agriculture and Forestry,Yangling 7 12100,China);EFFECT OF PAPRIKA EXTRACT SUPPLEMENT ON EGG YOLK COLOUR OF LAYER FED WHEAT-BASED DIETS[J];Acta Zoonutrimenta Sinica;2005-02
4 ZHANG Xu JIANG Guitao WANG Xiangrong HU Yan LI Haobang DAI Qiuzhong(Animal Nutrition and Feeding Technology Department,Hunan Institute of Animal Science and Veterinary Medicine,Changsha 410131,China);Effects of Tea Polyphenols on Performance,Egg Quality and Yolk Cholesterol Content of Laying Hens[J];Chinese Journal of Animal Nutrition;2011-05
5 ZOU Feng-ping1,2,XIONG Ming1,YU Qiong-wei*1,TANG Ziyong2,FENG Yu-qi1 (1.College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072;2.Faculty of Material Science and Chemical Engineering,China University of Geosciences,Wuhan 430074);Determination of Cholesterol in Monocyte-derived Macrophages by High Performance Liquid Chromatography[J];Journal of Analytical Science;2007-06
6 Mao Xinwu1, Liu Min2, Yin Pinghe2, Zhao Ling1 (1. Guangzhou Center for Disease Control and Prevention, Guangzhou 510632;2. College of Life Science & Technology, Jinnan University, Guangzhou 100800, China);Status in Distinguishing and Detection of the Waste Edible Oil[J];Guangdong Chemical Industry;2009-09
7 HE Chun-mei (Guangxi Agricultural Vocational and Technical Institute,Nanning 530007,China);Analysis of cholesterol in egg yolk by direct saponification-colorimetric method[J];Guangdong Agricultural Sciences;2011-09
8 HUANG Dao Ping CHEN Jian Hong LAO Yan Wen ZHOU Lin MO Jian Guang (Guangxi Research Centre of Analysis,No.32 Xinghulu,Nanning 530022,P.R.China );Determination of Cholesterol in Export Moon Cake by HPLC UV Detector[J];Chinese Journal of Spectroscopy Laboratory;2001-04
9 JIAO Yun-peng (Jiangsu Food Science College, Huaian 223003, China);Distinguishing and Detection of Waste Oils[J];Modern Food Science and Technology;2008-04
10 Gao Shiping Wu Liming(Faculty of Information Engineering,Guangdong University of Technology);Hogwash Oil Detection and Identification Portable Method Based on Embedded System[J];Automation & Information Engineering;2012-01
China Proceedings of conference Full-text Database 1 Hits
1 HAO Peng-fei *,MU Zhi-chun,XU Qin,ZHANG Xue-yan,LIU Lin,ZHAO Hua-mei (FATA of Shandong Entry-Exit Inspection and Quarantine Bureau,Qingdao 266002,China);Determination of Cholesterol in Edible Oil by GC-MS[A];[C];2012
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Huang Dezhi et al (Teaching Affairs Office, Anhui Agricultural University, Hefei 230036);Research Progress of Analysis of Pesticide Residue[J];Journal of Anhui Agricultural Sciences;2002-04
2 HOU Bo et al(Food Safety Research Base of Jiangsu Province,Jiangnan University,Wuxi,Jiangsu 214122);Literature Review of the Effects of Pesticide Residue on Agri-food Safety,Farmers'Perception on Pesticide Residue and the Influencing Factors[J];Journal of Anhui Agricultural Sciences;2010-04
3 ZHENG He et al(School of Chemical Engineering,University of Science and Technology,Liaoning Province,Anshan,Liaoning 114051);Determination of Five Phthalates in Instant Noodles Packaging[J];Journal of Anhui Agricultural Sciences;2011-19
4 LIU Jia-di et al(College of Chemistry and Chemical Engineering,Nanjing University of Technology,Nanjing,Jiangsu 210009);Determination of Cholesterol Content in Waste Fat by Dual-wavelength Spectrophotometry[J];Journal of Anhui Agricultural Sciences;2012-18
5 Liu Yizi Xie Mengxia Han Jie Liu Yuan Qiu Yueming Ding Yayun Zhang Lei (Analytical and Testing Center, Beijing Normal University, 100875, Beijing, China);DETERMINATION OF CHLORAMPHENICOL RESIDUE IN HONEY BY COUPLING SOLID-PHASE EXTRACTION WITH GAS CHROMATOGRAPHY-MASS SPECTROMETRY[J];Journal of Beijing Normal University(Natural Science);2004-02
6 ZHOU Xiang-juan,ZHAO Yu-qi,LI Wei,XIE Jing-jing(Haidian District Institute of Products Quality Supervision and Inspection,Beijing 100094,China);Determination of sixteen kinds of phthalic ester in cosmetics by gas chromatography-mass spectrometry[J];China Surfactant Detergent & Cosmetics;2010-02
7 DONG Wei-feng1,XU Yang1,CHEN Xi2,BAI Ling1,MU Ming-ren1,ZHANG Cui-fen31.Liaoning Entry-Exit Inspection and Quarantine Bureau,Dalian 116001,China;2.Dalian Entry-Exit Inspection and Quarantine Bureau,Dalian 116400,China;3.Great Wall Enterprise Company Limited,Dalian 116308,Chin;Determination of 6 kinds of phthalate acid esters in cosmetics by GC-MS[J];Journal of Dalian Polytechnic University;2012-04
8 Zou Yong-de,Zhou Yang(Nanhai Entry-Exit Inspection and Quarantine Bureau,Nanhai,Guangdong,528200,China);The method of Pre-treament and Determination of Phthalic Acid Esters in Food Containing Fat by GC-MS[J];Fujian Analysis & Testing;2012-03
9 Deng Hua, Zhu Pengling * (Department of Chemistry, Lanzhou University, Lanzhou 730000);Solid-phase Microextraction and Its Coupling with Other Analytical Technologies[J];Chinese Journal of Analytieal Chemistry;2001-05
10 Bao Zhongding *1 , Xu Rongnian 1, Zhang Songhong 2 1(Quality Inspection Center, Zhejiang Province Light Industry Institute, Hangzhou 310009) 2(Chemical Engineering College, Zhejiang University of Technology, Hangzhou 310014);Determination of Phytosterol and Cholesterol in Oil by Capillary Gas Chromatography[J];Chinese Journal of Analytieal Chemistry;2002-12
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Song Huifang,et al Analyzing And Computing Center;QUANTIFICATION OF CHOLESTEROL CHOLELITHLASIS BY FT-IR SPECTROSCOPY[J];;1992-01
2 LIU Wei~1,YIN Ping-he~1,ZHAO Ling~1,ZHANG Xuan~2,YU Han-hao~2,SHAN Xi-zhang~2,WANG Guo-cui~2 (1.College of Life Science & Technology,Jinnan University,Guangzhou510632,China; 2.Environment Protection Burean of Yuexiu District,Guangzhou510170,China);Distinguishing Hogwash Fat through Conductivity Measurement[J];Urban Environment & Urban Ecology;2004-03
3 Chen Jian-Bo,Liu Wei,Cui Yan-Mei,Zhao Dao-Yuan,Yang Ming-Min(College of Science,Nanjing Agriculture University,Nanjing 210095);Cloud Point Extraction for the Determination of Pesticides in Strawberry Juice by High Performance Liquid Chromatographic Detection[J];Chinese Journal of Analytical Chemistry;2008-03
4 Ma Yue Yan Zhe Huang Junxiong  (Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China);Cloud-Point Extraction and Its Applications to the Separation and Analysis of Biopolymers[J];Progress In Chemistry;2001-01
5 Shen Jinchao~1 Shao Xueguang~(1,2*)(1.Department of Chemistry,University of Science and Technology of China,Hefei 230026,China;2.Department of Chemistry,Nankai University,Tianjin 300071,China);Cloud Point Extraction and Its Applications to the Separation and Analysis of Organic Components[J];Progress in Chemistry;2006-04
6 WANG Li hong, GAO Zhi guang (Institute of Special Wild Economic Animal and Plant,CAAS,Jilin 132109);The Pharmacology Activities and the Clinical Applied in the Water Soluble Coronet Composition[J];JOURNAL OF ECONOMIC ANIMAL;1999-03
7 Fan Yulin;STUDY ON THE STEROIDS IN PILOSE ANTLER[J];Journal of Jilin Agricultural University;1987-02
8 Yang Yinkang1) Zhang Yuzhu1) Zhan Eryi1) Yang Haiming2) Liu Bo1)( 1) Department of Chemistry, Kunming Medical College;2) Department of Physics, Kunming 650031);Bilirubin Determination of Human Biliary Calculus by Spectophotometry[J];ACADEMIC JOURNAL OF KUNMING MEDICAL COLLEGE;1998-02
9 Yang Yinkang 1) Zhang Yuzhu 1) Zhang Eryi 1) Yang Haiming 2) Zhen Ping 3) ( 1) Department of Chemistry,Kunming Medical College;2)Department of Physics,Kunming 650031;3)The Center of Determination,Yunnan Normal University,K;Determination of Cholesterol in Human Biliary Calculus by TLc Scanning[J];ACADEMIC JOURNAL OF KUNMING MEDICAL COLLEGE;1998-04
10 LIANG Pei,LI Jing(College of Chemistry,Central China Normal University,Wuhan 430079, China);PROGRESS OF THE APPLICATION OF CLOUD POINT EXTRACTION TO THE SEPARATION AND ENRICHMENT OF METAL IONS AND TO THE SPECIATION ANALYSIS[J];Physical Testing and Chemical Analysis(Part B:Chemical Analysis);2006-07
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