A study of polymorphisms of five Y-chromosome STR loci in Han population in Zhejiang
WU Wei-wei, ZHENG Xiao-ting, HAO Hong-lei,et al.(Department of Criminal Investigation, Zhejiang Provincial Public Security Bureau, Hangzhou 310009,China)
ObjectiveTo complete the investigation of genetic polymorphism of Y-chromosome STR loci of Han population in Zhejiang, and provide the basal data for its forensic application. MethodDYS389Ⅰ、DYS389Ⅱ、DYS439、DYS438 and DYS392 loci were amplified by using Y-PLEX~(TM)5 PCR Amplification Kit and the products were analyzed by 3100 genetic analyzer, 200 unrelated male individuals of Han population in Zhejiang were investigated to determine the distributions of allele frequencies and haplotypes. (Results) 4、7、5、4、6 alleles were found in 5 Y-STR loci and the GD value were 0.5868、0.7779、0.6734、0.5062 and 0.6328 respectively. 95 haplotypes were found in 5 Y-STR loci, of which 54 were unique, 15 were found in 2 individuals,12 were found in 3 individuals,5 were found in 4 individuals,4 were found in 5 individuals, 1 were found in 6 individuals, 1 were found in 7 individuals, 2 were found in 8 individuals, 1 were found in 11 individuals, and the observed haplotypes diversity value was 0.9920. ConclusionThe 5 Y-STR loci are highly polymorphic and are suitable for forensic application in Zhejiang.