The role of bcl-2 gene in apoptosis of liver in rat with obstructive jaundice
WANG Jian Ming, ZOU Qian and ZOU Sheng Quan Department of Surgery, Tongji Hospital, Tongji Medical University, Wuhan 430030, Hubei Province, China
AIM To explore the mechanism of hepatic injury in obstructive jaundice (OJ). METHODS Forty adult male Wistar rats ( 190*!g - 230*!g ) were used in all experiments. All rats underwent laparotomy. Experimental obstructive jaundice (BDL) was induced by double ligation of the bile duct by a proximal ligature around the bile duct in the hilus of the liver and by a distal ligature close to its entry into the duodenum. The animals were randomly divided into two groups: those that underwent bile duct manipulation (sham operated) and those that underwent BDL for 3, 7, 14, and 21 days. We detected the serum bilirubin and pathological change of liver in all rats. Then we detected apoptotic status and the expression of apoptosis related bcl 2 gene protein in liver of all rats with terminal deoxynucleotidyl mediated nick end labeling (TUNEL) and immunohistochemistry method. RESULTS The apoptotic rate of liver is related to times of OJ. Apoptosis was highest 14 days after bile duct ligation, apoptotic index (AI) was 58 23±1 58 ( P 0 05) . The stronger the bcl 2 expression, the less number of apoptotic cells in OJ. The increase of bilirubin and pathological change of liver in OJ were obvious. CONCLUSION bcl 2 takes part in the regulation and occurrence and progression of hepatic injury in OJ.
【CateGory Index】： R575