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《World Chinese Journal of Digestology》 2000-01
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Study on the apoptosis and cell cycle arrest in human liver cancer SMMC7721 cells induced by Jianpiliqi herbs

Wei Jian Guo, Er Xin Yu, Song Guo Zheng, Zhao Zhong Shen, Jian Ming Luo, Guo Hua Wu and Shi An Xia Department of Radiotherapy, Xinhua Hospital of Shanghai Second Medical University, Shanghai 200092, China Cancer Hospital of Shanghai Medical University, Shanghai 200032, China Correspondence to: Wei Jian Guo, Department of Radiotherapy, Xinhua Hospital of Shanghai Second Medical University, Shanghai 200092, China  
AIM To evaluate the inducing effects of Jianpiliqi herbs on apoptosis and to offer experimental basis for its clinical application.METHODS The effects of Jianpiliqi herbs were investigated in vitro using serum pharmacological approach. The inhibiting effect of Jianpiliqi herbs containing rabbit serum on SMMC7721 cells was observed using tetrazolium dye (MTT) assay. Apoptosis and cell cycle arrest induced by Jianpiliqi herbs were determined with Annexin V-affinity assay, DNA content analysis and electron microscopy. Effects of Jianpiliqi herbs on p53 and P21WAF1/CIP1 protein expression were assessed by cell culture and immunohistochemistry. With RT-PCR assay, changes in expression of P21WAF1/CIP1 mRNA after Jianpiliqi herbs treatment were observed.RESULTS The serum containing Jianpiliqi herbs could inhibit the proliferation of cancer cells. The inhibiting rate was 6.6% for 3-day treatment and 36.2% for 6-day treatment. The apoptosis rate in SMMC7721 cells induced by Jianpiliqi herbs was 9.8%±4.0%. Jianpiliqi herbs arrested cell cycle at S phase, and increased the expression of p53 protein, P21WAF1/CIP1 mRNA and protein. CONCLUSION Jianpiliqi herbs have the effects of inducing apoptosis and inhibiting the proliferation of livercancer cells. It can enhance the expression of p53 and p21wAF1/CIP1 gene, which might be the molecular mechanism.
【CateGory Index】: R735.7
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