Gene expression profiling in intestinal-type gastric carcinoma by cDNA microarray
Xin-Hua Li, Wan-Dai Zhang, Bin Xiao, Zhen-Shu Zhang, Department of Gastroenterology, The First Military Medical University, Guangzhou 510515, Guangdong Province, China Xin-Hua Li, Department of Gastroenterology, The Fourth Military Medical University, Xi'an 710032, Shanxi Province, China Bo-Tao Wang, Department of Burn, The Fourth Military Medical University, Xi'an 710032, Shanxi Province, China
AIM: To identify a set of genes involved in the development of intestinal-type gastric carcinoma. METHODS: Pure mRNAs from 6 cases of intestinal-type gastric carcinoma and corresponding noncancerous mu-cosae were reversely transcribed into cDNAs labeled with Cy5 and Cy3 dyes for probes, then mixed and hybridized with the cDNA microarray consisting of 4 096 genes, and the fluorescent signals were scanned. RESULTS: Among total genes, 333 were up-regulated and 333 down-regulated in intestinal-type gastric cancer tissues. Within altered expression of those genes, cell-cycle regulators and growth factors were up-regulated, and the promoter genes of apoptosis were down-regulated; Oncogenes and cell-adhesion molecules were more up-regulated; The cancer progression genes were up-regulated, while the anti-cancer progression genes were down-regulated. CONCLUSION: The quick and high-throughout method of gene expression profile by cDNA array provides us with an overview of gene changes that may involved in intestinal-type gastric cancer development, and will open up new possibilities to identify novel molecular targets for diagnosis and therapy. Several genes are altered in intestinal-type gastric cancer, which need to be further investigated.
【CateGory Index】： R735.2;