Expression and signif icance of phosphorylated focal adhesion kinase in colon carcinom
Qi-Sheng Liu, Hong-Gang Yu, Yuan-Ling Qi, Jun Cao, He-Sheng Luo, Jie-Ping Yu, Department of Gastroenterology, People’s Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
AIM: To investigate the expression of phosphorylated focal adhesion kinase (phospho-FAK) and its signifi c- ance in human colon carcinoma. METHODS: The phospho-FAK (including FAK) expre- ssion was detected by Western bloting in 20 cases of colon carcinoma and their corresponding para-cancer tissues. RESULTS: The positive rate of FAK expression in the cancer tissues was signif icantly higher than that in thecorresponding normal tissues (95% vs 60%, c2 = 5.16, P 0.05). The mean level of FAK expression in the cancer tissues was 0.482±0.150, while the mean level of expression in the normal tissue was 0.269±0.015 (t = 6.39, P 0.01). The positive rate of Tyr-397 FAK protein expression in the cancer tissues was 90%, while the positive rate in the corresponding normal tissues was only 20% (c2 = 17.1, P 0.01). The mean level of Tyr-397 FAK protein expression in the cancer tissue was nota- bly higher than that in the corresponding para-cancer tissues (0.385±0.021 vs 0.110±0.005, t = 54.23, P 0.01). CONCLUSION: The up-regulation of FAK expression, especially Tyr-397 FAK protein expression, may play an important role in the tumorigenesis and progression of colon carcinoma.