Killing effect of oxymatrine on human gastric cancer cell line MKN45 and its mechanism
Xiao-Feng Yu Jian Zou Department of Digestology,Shanghai Huadong Hospital,Shanghai 200040,China Zhi-Hua Ran Department of Digestology,Shanghai Renji Hospital,Shanghai 200001,China
AIM:To explore the killing effects of oxymatrine (OM)on human gastric cancer cell line MKN45 and its anti-neoplastic mechanism. METHODS:Human gastric cancer cell line MKN45 was cultured and then treated with 0.5, 1,2,4 and 6 g/L OM.Methylthiazolyl tetrazo- lium analysis(MTT)was used to observe the killing effect of OM on MKN45 cells.Cell cycle distribution was measured by flow cytometry. The activity of telomerase was detected by poly- merase chain reaction(PCR)-enzyme linked im- munosorbent assay(ELISA).Reverse transcrip- tion(RT)-PCR was employed to examine the expression of hTERT,c-myc,p53 and mad1 genes in MKN45 cells. RESULTS:OM exhibited dose-dependent killing effects on MKN45 cells and its IC_(50)was 2.78 g/L. After administration for 48 hours,OM induced an increase of G1/G0-phase cells(62.2%±1.3% vs 56.7%±4.0%,P0.05)and decrease of G2/ M-phase cells(5.4%±1.1% vs 10.0%±2.8%,P0.05)at a dose of 2 g/L,and a decrease of S-phase cells(30.5%±1.3% vs 33.4%±1.2%,P0.05)at a dose of 4 g/L.OM inhibited the activity of telom- erase in MKN45 cells in a dose-dependent man- ner.The expression of hTERT gene in MKN45 cells was decreased,but the expression of p53 and mad1 genes were increased.However,and c-myc gene expression had no apparent changes. CONCLUSION:OM has dose-dependent kill- ing effects on MKN45 cells,and it can inhibit the telomerase activity through hTERT gene and the up-stream regulation genes.
【CateGory Index】： R735.2