Sequence variability of human cytomegalovirus UL147 genes in clinical isolates
Rong He,Qiang Ruan,Ying Qi,Yan-Ping Ma,Virus Laboratory,Shengjing Hospital of China Medical Univer-sity,Shenyang 110004,Liaoning Province,China
AIM:To explore the sequence variability of human cytomegalovirus (HCMV) UL147 ORF in clinical isolates and its possible associations with HCMV infection.METHODS:HCMV-UL147 ORF was amplified by PCR assay and sequenced in 23 low-passage isolates and urine from two healthy children. The whole gene sequence of HCMV-UL147 in the urine of healthy children was determined and analyzed.RESULTS:The length of UL147 ORF of 25 isolates was 464-480 bp. Sequencing analysis showed significant strain-specific variability,and none of the sequences was exactly the same with Toledo. The variation was located in the 5' end of sequence. The prediction of secondarystructure demonstrated two types and the amino acid sequences kept highly conservative in the important functional area of protein. In jaundice,microcephaly and Hirschsprung's disease,no sequence differences were found among the disease types.CONCLUSION:HCMV-UL147 might play a role in HCMV infection and subsequent diseases.