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《World Chinese Journal of Digestology》 2012-04
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Sequence polymorphisms of the UL147 gene in clinical strains of human cytomegalovirus

Xiao-Bo Yin,Zhi-Qin Mao,Shu-Ting Chang,Mei Sun,Department of Pediatric Gastroenterology,Shengjing Hospital,China Medical University,Shenyang 110004,Liaoning Province,China Yao-Hua Ji,Rong He,Ying Qi,Qiang Ruan,Yan-Ping Ma,Zheng-Rong Sun,Virus Laboratory,Shengjing Hospital,China Medical University,Shenyang 110004,Liaoning Province,China  
AIM:To investigate the sequence polymorphisms in the open reading frame(ORF) of the UL147 gene in clinical strains of cytomegalovirus and to identify the relationship between UL147 sequence polymorphism and cytomegalovirus hepatitis.METHODS:The ORF of the UL147 gene in 19cytomegalovirus strains isolated from infants with cytomegalovirus hepatitis was amplified by PCR and sequenced.RESULTS:In comparison with the sequence of the Toledo strain,strain-specific polymorphisms were present in the 5' end of the UL147 ORF,and the polymorphisms were located in the 5' end of sequence.The UL147 ORF varied from 477-480 kb.No sequence differences were found between strains isolated from infants with cytomegalovirus hepatitis and asymptomatic patients.CONCLUSION:The ORF of the UL147 gene is hypervariable in different cytomegalovirus strains.The conservation of predicted UL147 product in clinical strains suggests that UL147 gene polymorphisms have no relationship with the severity of cytomegalovirus hepatitis.
【Fund】: 国家自然科学基金资助项目 No.30700916~~
【CateGory Index】: R725.7
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