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《Journal of Xinxiang Medical College》 2011-06
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Determination of puerarin in Shiwei Jiangtang effervescent granules by high performance liquid chromatography

DU Wei-qi1,SHI Xiu-fang2,ZHANG Wen-bo3,ZHANG Zhen-zhong2(1.Drug and Equipment Section,Anyang Regional Hospital,Anyang 455000,Henan Province,China;2.School of Pharmaceutical Science,Zhengzhou University,Zhengzhou 450001,Henan Province,China;3.Department of Life Science and Technology,Xinxiang Medical College,Xinxiang 453003,Henan Province,China)  
Objective To establish a quantitative analysis method of puerarin in Shiwei Jiangtang effervescent granules by high performance liquid chromatography(HPLC).Methods HPLC with Hypersil C18 column(250.0 mm×4.6 mm,5.0 μm) was used,methanol-0.025 mol·L-1 phosphate(pH 3.5)(14) as the mobile phase,at the detection wavelength of 250 nm and the flow rate of 1.0 mL·min-1.Results The number of theoretical plates of the column was 7 600,calculated with reference to the peak of puerarin.The puerarin of test sample was well separated from other impurities with separating degree R1.5,trailing factor was 1.The calibration curve of puerarin was a good linear relationship in the range of 2.515-100.600 mg·L-1(r=0.999 9).The average recovery rate was 98.58%,and rlative standard deviation was 2.40%.Conclusion This method is accurate,reliable and can be used for the determination puerarin in Shiwei Jiangtang effervescent granules.
【CateGory Index】: R286.0
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