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《Journal of Xinxiang Medical College》 2012-08
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Effects of bone marrow mesenchymal stem cells and its supernate on rats with cerebral infarction

ZHAO Xiao-mei,LIU Yong-gang(Department of Neurology,the First Central Hospital of Baoding City,Baoding 071000,Hebei Province,China)  
Objective To explore the effect of bone marrow mesenchymal stem cells(BMSCs) and its supernate on rats with cerebral infarction.Methods Forty rats were randomly divided into sodium chloride injection group(9 g·L-1 sodium chloride injection 2 mL),BMSCs transplantation group(2.0×109 L-1 BMSCs suspension 2 mL),BMSCs supernate transplantation group(BMSCs supernate 2 mL) and suspension transplantation group(supernatant and BMSCs suspension 2 mL);ten rats in each group.Twenty-four hours after reperfusion,the endogenous neural precursor cells were labeled by 10 g·L-1 bromodeoxyuridine(BrdU) solution for twenty-eight days,the rats were executed after the last injection two hours.The number of neural precursor cells of multiplication period and neurological severity scores(NSS) were observed in each group.Results The positive cells labeled by BrdU,BrdU+neurone specific enolase,and BrdU+glial fibrillary acidic protein(GFAP) in BMSCs transplantation group,BMSCs supernate transplantation group and suspension transplantation group were more than those in sodium chloride injection group(P0.05);and the positive cells in BMSCs supernate transplantation group and suspension transplantation group were more than those in BMSCs transplantation group(P0.05);and the positive cells in suspension transplantation group were more than those in BMSCs supernate transplantation group(P0.05).Compared with pretransplant,at the seventh,fourteenth and twenty-eighth day after transplantation,the nerve function of rats recovered in different degrees,and the NSS scores were decreased gradually,the differences had statistical significance(P0.01).Starting from the seventh day,the NSS score in BMSCs transplantation group,BMSCs supernate transplantation group and suspension transplantation group was significantly lower than that in sodium chloride injection group(P0.05 or P0.01);the NSS score in suspension transplantation group was significantly lower than that in BMSCs transplantation group and BMSCs supernate transplantation group(P0.05).Conclusion The supernatant and suspension of BMSCs can induce the proliferation and differentiation of endogenous neural precursor cells in focal zone,which may be related to the neurotrophic factors and cellular differentiation inducing factor in supernatant induced BMSCs differentiated into neural cell.
【CateGory Index】: R743.3
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