Determination of Six Phendic Acids in Mailuoning Injection by RP-HPLC
ZHANG Hui1, LI Xiang1, ZHOU Hong-yan2, et al (1.Nanjing University of TCM, Nanjing 210046, China;2.Technology Center of Jinling Pharmaceutical Company, Nanjing 210009, China)
Objective To establish a method for determination the contents of six phendic acids in Mailuoning injection by HPLC at same time. Methods Separation was performed on a Kromasil-C18 (4.6 mm× 250 mm, 5 μm) column with mobile phase consisting of 0.1% phosphoric acid-water and Acetonitrile with gradient elute at the flow rate of 1 mL/min. The UV detection wavelength was set at 327 nm and the column temperature was set at 25 ℃. The sample size was 20 μL. Results The standard cures of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, 3,5-Dicaffeoylquinic acid, 3,4- Dicaffeoylquinic acid were linear within the contentration range of 0.010 90～0.544 8 μg (r=0.999 9), 0.024 06～1.203 2 μg (r =1), 0.011 06～0.552 8 μg (r =1), 0.015 94～0.796 8 μg (r =1), 0.009 104～0.455 2 μg (r =1), 0.010 37～0.518 4 μg (r=1) respectively. And the recovery rates of them were 101.06%, 100.22%, 101.54%, 99.42%, 100.21%, 101.65% respectively. Conclusion The method appeared to be simple, reliable, accurate and can be used to determine the contents of six phendic acids in Mailuoning injection.
【CateGory Index】： R286