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Effect of Danlong Xingnao Tablet on the Expression of TNF-α and Apoptosis after Focal Cerebral Ischemia Reperfusion in Rat Hippocampus

HE Qian, ZHOU Xiao-qin, LI Hua, et al (Hunan University of TCM,Changsha 410007, China)  
Objective To study the effects of Danlong Xingnao Tablet on neuronal apoptosis and expression of TNF-α after focal cerebral ischemia reperfusion in rat hippocampus. Methods The model of middle cerebral arteries occlusion (MCAO) were performed with intraluminal filament blockade. By using in situ terminal deoxynucleotidyl transferase-mediated dUTP-biotor nick end labeling (TUNEL) and immunohistochemistry staining, the number of apoptotic cells and TNF-α positive cells were detected in the sham-operated group, the ischemia reperfusion group, Danlong Xingnao Tablet group and nimodipine group after the middle cerebral arteries of SD rats occluded for 1 h and reperfused for 24 h.Results Compared with the sham operation group, the number of apoptotic cells and TNF-α positive cells of the model group increased significantly (P 0.05, P 0.01). Compared with the model group, DLXNT intragastric administration could reduce the number of apoptotic cells and TNF-α positive cells (P0.05). Conclusion The decreased expression of TNF-α may be one of the molecular mechanisms of decreased apoptotic cell after rat cerebral ischemia reperfusion injurying treated with Danlong Xingnao Tablet.
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