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《Journal of Xianyang Normal University》 2008-02
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Detection of the Content of the Puterrin in Xiao'er Qingfei Zhike Pian with the HPLC Method

ZHANG Ping,WANG Xiao-ling,CHEN Yan,CHEN Xiao-hui(Department of Chemistry,Xianyang Normal University,Xianyang,Shaanxi 712000,China)  
A method has been developed for determination of puterrin in Xiao'er Qingfei Zhike Pian by High Performance Liquid Chromatography.Sample was prepared through Ultrasonic Wave enhancing extraction puterrin for 15min in ethanol.The separation was performed on an Shim-Pack VP-ODS column(250mm ×4.6mm,5μm) with a mobile phase of methanol and water(30:70) and a detection wavelength of 250nm.The range of linearity was from 1.0 mg·L-1 to 50.0 mg·L-1(r=0.999 9).The lower limit of detection of puterrin was 0.017 mg·L-1,The average recovery was 98.80% and RSD of the recurrence was 3.48%(N=5).The results showed that the method is simple,rapid,accurate and reproducible.
【Fund】: 咸阳师范学院科研基金项目(04XSYK203)
【CateGory Index】: R286.0
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