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《Journal of Experimental Hematology》 2009-04
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Detection of APC Gene Promoter Methylation in Hematological Malignant Cell Lines by Nested-Methylation Specific Polymerase Chain Reaction

WU Xue-Mei,SHEN Jian-Zhen,YU Ai-Fang,FAN Li-Ping,ZHOU Hua-Rong,FU Hai-Ying,SHEN Song-Fei,WU Dan-SenFujian Institute of Hematology,Union Hospital,Fujian Medical University,Fuzhou 350001,Fujiam Province, China  
This study was aimed to investigate the effeciency of nested methylation specific polymerase chain reaction (nMS-PCR) for detecting the APC gene promoter methylation and to clarify the roles of methylation in genesis and development of hematologic malignancies, as well as to screen the hematologic malignant cell lines with hypermethy- lation of APC gene promoter to use as an ideal cell model for exploring the relationship between gen methylation and gene expression. The genome DNA of 10 cell lines modified with bisulfide was amplified and the methylation status of APC gene promoter was detected by using nMS-PCR. The results showed that the methylation of APC gene promoter was detected in Jurkat cells, while could not be detected in CA46, U266, Molt4, K562, HL-60, CEM, AKR, U937 and Raji cell lines.In conclusion, APC gene methylation in hematological malignant cell lines can be accurately detected by nMS-PCP method, which is simple method for detecting methylation status of various hematological malignant cell lines.
【Fund】: 福建省自然基金资助项目(编号C0540014);; 福建省百千万人才工程基金资助项目(编号303052801);; 福建医科大学教授基金(编号JS06080)资助项目
【CateGory Index】: Q75
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