Detection,Diagnosis and Analysis of the First Case of Neonatal Alloimmune Thrombocytopenia Purpura Associated with AntiHPA-5b in China
ZHOU Yan;ZHONG Zhou-Lin;LI Li-Lan;SHEN Wei-Dong;WU Guo-Guang;Nanning Institute of Transfusion Medicine,Nanning Blood Center;
This study was aimed to investigate the detection and diagnosis of the neonatal alloimmune thrombocytopenia purpura( NAITP) caused by anti-HPA-5b antibody. The platelet count and clinical manifestation in the newborn were examined. The HPA-1- 21bw genotypes of the newborn and her parents were detected by multiplePCR and DNA sequencing. The HPA-specific antibody in the sera of newborn and her mother were detected and identified by flow cytometry( FCM) and monoclonal antibody-specific immobilization of platelet antigens( MAIPA). The results indicated that the clinical manifestations of the newborn were lighter. The HPA genotyping showed that the genotype of the newborn was HPA-5ab,while that of her mother and father were HPA-5aa and HPA-5ab,respectively. The antibody against the platelet of newborn' s father existed in the newborn' s mother sera. The HPA antibody of the mother was identified as anti-HPA-5b. It is concluded that the newborn with neonatal alloimmune thrombocytopenia purpura was caused by the antibody against HPA-5b.