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《China Occupational Medicine》 2006-03
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The effect of alveolar macrophage inhibition in perfluoroisobutylene-induced acute lung injury

ZHAO Min,CHEN Jia-bin,WANG He-mei,et al(Beijing Institute of Pharmacology and Toxicology,Beijing 100850,China)  
Objective To investigate preliminarily the role of alveolar macrophage(AM) in perfluoroisobutylene(PFIB)-induced acute lung injury.Methods 248 male KM mice were randomly divided into four groups(namely control group,GdCl_3 group,PFIB group and GdCl_3/PFIB group),among which the PFIB and GdCl_3/PFIB groups were exposed to PFIB at the concentration of 130 mg/m~3 for 5 minutes in a flow-past whole-body chamber,while the control and GdCl_3 groups were exposed to the filtered air in a similar manner.Before PFIB or filtered air exposure,the GdCl_3 and GdCl_3/PFIB groups were pretreated with GdCl_3 intravenously(10 μg/g) at 48 hours and 24 hours,respectively,and the control and PFIB groups were injected the same dose of normal saline at the corresponding time-points.The content of total protein in brochoalveolar lavage fluid(BALF) and the lung wet-to-dry weight ratio was determined at 8,12,24,48 and 72 hours post PFIB exposure,with histopathological and(ultrastructural) examination being done at 24 hours and the mortality rate induced by PFIB exposure at 190 mg/m~3 for 5 minutes within the 7-day observation period being recorded.Results When AM inhibition was achieved by GdCl_3 pretreatment,death of mice induced by an over-LCt_(50) concentration of PFIB(at 190 mg/m~3 for 5minutes) dropped dramatically,which was well supported by the amelioration in histopathological and ultrastructural changes observed at 24 hours post PFIB exposure as well as significant declination in content of BALF total protein and lung wet-to-dry weight ratio observed at 8,12 and 24 hours post exposure,(respectively) in the GdCl_3/PFIB group.Conclusion AM plays an important role in the pathogenesis of PFIB-induced acute lung injury,the underlying mechanism of which needs to be further clarified.
【Fund】: 国家自然科学基金资助项目(30300280);; 国家教育部博士点基金课题(20010001092)
【CateGory Index】: R563
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【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Hu 1,2,WANG He-mei 1,ZHAO Jian 1,DING Ri-gao 1,HUA Zi-chun 2;Pathogenesis of perfluoroisobutylene inhalation-induced acute lung injury[J];China Occupational Medicine;2013-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 XIE Yan ping 1, WANG Jian chun 1, QIAN Gui sheng 1, WANG Ying deng 2, XIAO Zhen liang 1 ( 1Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037; 2Department of Respiratory Diseases, Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China);Effects of lipopolysaccharide on aquaporin-1 expression in cultured rat lung microvessel endothelial cells[J];Acta Academiae Medicinae Militaris Tertiae;2004-09
2 XU Shun-gui~(1),WU Guo-ming~(1),XU Zhi~(1),FENG Qi-jia~(1),WANG Guan-song~(1),ZHANG Jian-peng~(2)(~(1)Institute of Respiratory Diseases,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,~(2)Department of Respiratory Diseases,General Hospital of Chinese People's Armed Police Forces,Beijing 100039,China);Comprehensive characterization of rat pulmonary microvascular endothelial cells cultured by tissue-sticking method[J];Acta Academiae Medicinae Militaris Tertiae;2007-01
3 DAI Lie,TANG Meian,YIN Peida,et al.Third Affiliated Hospital of Sun Yat sen University of Medical Sciences,Guangzhou 510630;Relationship between synovial macrophage and disease activity or articular damage in rheumatoid arthritis[J];CHINESE JOURNAL OF RHEUMATOLOGY;1999-04
4 DAI Lie ,YE Zhiqiang,TANG Meian,et al. Department of Rheumatic Immunology,Third Affiliated Hospital of Sun Yat sen University of Medical Sciences,Guangzhou 510630, China;Research on source of macrophage in rheumatoid synovium[J];CHINESE JOURNAL OF RHEUMATOLOGY;2000-02
5 XIONG Zhuang*, YU Yong-qiang, L?譈 Yi-zhong, QIAN Yin-feng, YUE Li, JIANG Shi-ping, CHEN Yang,TIAN Yu-lian. *The Department of Radiology of the Affiliated Hospital of Anhui Medical University, Hefei 230022, China;In vivo evaluation of early disease progression by X-ray phase contrast imaging in the adjuvant arthritis rat[J];Chinese Journal of Rheumatology;2004-08
6 ZHANG Xiangfeng, Gao Mingzhe, Hussein D. FodaDepartment of Pulmonary Medicine, Beijing Anzhen Hospital, Beijing 100029, China(ZHANG Ziangfeng,GAO Mingzhe);Expression of matrix metalloproteinases 2 and 9 in mice with hyperoxia-induced acute lung injury[J];Journal of Emergency Medicine;2005-01
7 LI Tao~* XIAO Yu WANG Hong-wei MAI Gen-rong XU Shao-yong XIE Ji-jian LI Dong-sheng. *Medical Center of Pediatrics, Affiliated Taihe Hospital, Yunyang Medical College, Shiyan 442000, China;Effect of vascular endothelial growth factor on synthesis of pulmonary surfactant[J];Chinese Journal of Emergency Medicine;2007-10
8 MENG Ge1,ZHAO Jian1,L Xin-Huai2,DING Ri-Gao1(1.Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing,100850,China;2.Longkou People's Hospital,Longkou,Shandong 265701,China);Improvement of rat pulmonary microvascular endothelial cell primary culture methods[J];Bulletin of the Academy of Military Medical Sciences;2009-06
9 WANG He-mei,HUANG Chun-qian,ZHANG Xian-cheng,QU Wen-sheng,SUN Xiao-hong, DING Ri-gao,RUAN Jin-xiu(National Beijing Center for Drug Safety Evaluation and Research,Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China);The time-course effect of perfluoroisobutylene inhalation on endogenous nitric oxide and its synthases activity in rats[J];Bulletin of the Academy of Military Medical Sciences;2010-06
10 HUANG Xing Xu, CHEN Lian Bo 1) , BAO Yong Yao 1) , PIAO Ying Jie 1) , HUANG You Guo(National Laboratory of Biomacromolecules, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China; 1) Central Laborato;Study on the Apoptosis of Macrophages Induced by Dexamethasone.[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;2000-05
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