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《China Occupational Medicine》 2006-03
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The effect of alveolar macrophage inhibition in perfluoroisobutylene-induced acute lung injury

ZHAO Min,CHEN Jia-bin,WANG He-mei,et al(Beijing Institute of Pharmacology and Toxicology,Beijing 100850,China)  
Objective To investigate preliminarily the role of alveolar macrophage(AM) in perfluoroisobutylene(PFIB)-induced acute lung injury.Methods 248 male KM mice were randomly divided into four groups(namely control group,GdCl_3 group,PFIB group and GdCl_3/PFIB group),among which the PFIB and GdCl_3/PFIB groups were exposed to PFIB at the concentration of 130 mg/m~3 for 5 minutes in a flow-past whole-body chamber,while the control and GdCl_3 groups were exposed to the filtered air in a similar manner.Before PFIB or filtered air exposure,the GdCl_3 and GdCl_3/PFIB groups were pretreated with GdCl_3 intravenously(10 μg/g) at 48 hours and 24 hours,respectively,and the control and PFIB groups were injected the same dose of normal saline at the corresponding time-points.The content of total protein in brochoalveolar lavage fluid(BALF) and the lung wet-to-dry weight ratio was determined at 8,12,24,48 and 72 hours post PFIB exposure,with histopathological and(ultrastructural) examination being done at 24 hours and the mortality rate induced by PFIB exposure at 190 mg/m~3 for 5 minutes within the 7-day observation period being recorded.Results When AM inhibition was achieved by GdCl_3 pretreatment,death of mice induced by an over-LCt_(50) concentration of PFIB(at 190 mg/m~3 for 5minutes) dropped dramatically,which was well supported by the amelioration in histopathological and ultrastructural changes observed at 24 hours post PFIB exposure as well as significant declination in content of BALF total protein and lung wet-to-dry weight ratio observed at 8,12 and 24 hours post exposure,(respectively) in the GdCl_3/PFIB group.Conclusion AM plays an important role in the pathogenesis of PFIB-induced acute lung injury,the underlying mechanism of which needs to be further clarified.
【Fund】: 国家自然科学基金资助项目(30300280);; 国家教育部博士点基金课题(20010001092)
【CateGory Index】: R563
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1 WANG Hu 1,2,WANG He-mei 1,ZHAO Jian 1,DING Ri-gao 1,HUA Zi-chun 2;Pathogenesis of perfluoroisobutylene inhalation-induced acute lung injury[J];China Occupational Medicine;2013-03
Chinese Journal Full-text Database 10 Hits
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