Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《China Occupational Medicine》 2019-01
Add to Favorite Get Latest Update

Expression of IL-23 and IL-17 in immune injury of the liver of mice sensitized by trichloroethylene and aggravated by Poly I:C

CHEN Shuangping;ZHANG Cheng;XIE Haibo;LI Na;ZHANG Jiaxiang;WANG Feng;YANG Ling;ZHU Qixing;Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University;  
Objective To observe the expression of interleukin(IL)-23 and IL-17 in the liver of mice sensitized by trichloroethylene(TCE), and to explore the role of IL-23 and IL-17 in polyinosinic: polycytidylic acid(Poly I:C) exacerbated TCE-sensitized mice with immune injury of the liver. Methods Female specific pathogens free BALB/c mice were randomly divided into control group(n=5), solvent control group(n=5), TCE group(n=20), and TCE+Poly I:C group(n=20). A TCE-sensitized mouse model was established in TCE group and TCE+Poly I:C group. Three hours before the last challenge, mice in TCE+Poly I:C group was intraperitoneally injected with a mass concentration of 0.5 g/L poly I:C, 100 μL per mouse. The two groups of mice were divided into sensitized and non-sensitized subgroups according to the results of skin sensitization. After 48 hours of the final challenge, serum alanine aminotransferase(ALT) was detected by colorimetric method. The histopathological changes of mouse liver were observed, and the expression of IL-23 and IL-17 in liver tissues was detected by immunohistochemistry and Western blotting method. Results The sensitization rates of TCE and TCE+Poly I:C groups were 35.0%(7/20) and 40.0%(8/20) respectively, with no significantly statistical difference(P0.05). Pathological examination showed that there was cell edema in some areas of the liver tissues of mice in the TCE-sensitized subgroup, while the TCE+Poly I:C sensitized subgroup showed cell vacuolar degeneration and loose cytoplasm. Serum ALT activity and the expression of IL-23 and IL-17 in liver tissues in the TCE-sensitized subgroup were higher than that in the blank control group, the solvent control group and the TCE non-sensitized subgroup(P0.05). Serum ALT activity and IL-23 and IL-17 expression in the TCE+Poly I:C sensitized subgroup were higher than that in the TCE-sensitized subgroup(P0.05). The relative expression of IL-23 and IL-17 protein in liver tissues in TCE-sensitized subgroup was higher than that of the blank control group and the solvent control group(P0.05), while that in TCE+Poly I:C sensitized subgroup was higher than that of TCE-sensitized subgroup(P0.05). Conclusion IL-23/IL-17 axis may play an important role in the development of immune injury of liver in the TCE-sensitized mice and Poly I:C exacerbated TCE-sensitized mice.
【Fund】: 国家自然科学基金项目(81673141、81502791);; 安徽省教育厅高校自然科学研究项目(KJ2017A206)
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 5 Hits
1 YI Juan;LIU Zheng-yu;LI Tian-di;ZHANG Jian-jie;LIU Jian;LIN Yi-ran;LI Shuang-feng;Shenzhen Prevention and Treatment Center for Occupational Diseases /Shenzhen Key Lab for Technology of Diagnosis and Treatment of Occupation Disease;;Serum cytokines levels in patients with trichloroethylene induced medicamentosa-like dermatitis[J];职业卫生与应急救援;2015-02
2 LI Bai-xue;ZHANG Ji;WU Jiang;FENG Quan-sheng;ZHANG Chuan-tao;Chengdu University of Traditional Chinese Medicine;Affiliated Hospital of Chengdu University of Tranditional Chinese Medicine;;The Molecular Mechanism of Hepatic Cell Injury Model in Vitro[J];现代生物医学进展;2014-09
3 WANG Feng;ZHANG Jia-xiang;XU Shu-hai;JIANG Tao;YU Jun-feng;SHEN Tong;ZHU Qi-xing;Department of Occupational and Environmental,School of Public Health,Anhui Medical University;;Expressions of IL-6 in skin and serum of TCE-sensitized mice[J];中国职业医学;2014-01
4 LI Xiao-ni;DENG Zhi-hua;HE Jing;WANG Qi;Department of Gastroenterology,The Second Hospital of Shanxi Medical University;;The Role of IL-23/IL-17 in Experimental Autoimmune Hepatitis[J];中国医药指南;2013-24
5 WU Qi-feng,ZHAO Na,LI Cong,LIANG Wei-hui,DENG Xiao-feng,LI Bin,ZENG Zi-fang Guangdong Prevention and Treatment Center for Occupational Diseases,Guangzhou 510300,China;Correlation between lymphocyte subsets and liver dysfunction among patients with dermatitis medicamentosa-like of Trichloroethylene[J];职业卫生与应急救援;2013-01
【Co-citations】
Chinese Journal Full-text Database 8 Hits
1 LANG Li;FAN Chun-yue;WU Qi-feng;WANG Yan-yan;LAI Ming-zhen;ZHANG Ying;CHENG Ying;LIANG Wei-hui;Guangdong Province Hospital for Occupational Disease Prevention and Treatment;Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment;;Clinical analysis on three cases of occupational medicamentosa-like dermatitis due to trichloroethylene complicated with hyperthyroidism[J];中国职业医学;2015-06
2 Huang Peiwu;Li Xuan;Liu Wei;Liu Jianjun;Shenzhen Center for Disease Control and Prevention;;Advances in non-carcinogenic toxicity of trichloroethylene[J];中华预防医学杂志;2015-09
3 Liu Jianjun;Key Laboratory of Modern Toxicology of Shenzhen,Shenzhen Center for Disease Control and Prevention;;Strengthen the prevention of occupational trichloroethylene health hazards[J];中华预防医学杂志;2015-03
4 YANG Jin;FU Ping;Department of Dermatology,the Second Affiliated Hospital of Kunming Medical University;;Progress on Th_(17)/Treg cells in the pathogenesis of pemphigus vulgaris[J];皮肤病与性病;2015-01
5 WU Qi-feng;ZHAO Na;LI Cong;LIANG Wei-hui;ZENG Zi-ang;LI Bin;DENG Xiao-feng;Guangdong Hospital for Prevention and Treatment of Occupational Disease;;Changes of lymphocyte subsets and liver dysfunction in patients with dermatitis medicamentosa-like of trichloroethylene after treatment[J];职业卫生与应急救援;2014-06
6 JIA Qiang;JI Yu-qing;MENG Tao;ZHAO Xue-zheng;ZHANG Jun;ZHENG Yu-xin;DAI Yu-fei;Key Laboratory of Chemical Safety and Health,National Institute for Occupational Health and Poison Control,Chinese Center for Disease Control and Prevention;;The exploration of trichloroethylene-induced delayed hypersensitivity effect with HLA-B*1301 transgenic mice[J];中国职业医学;2014-05
7 XU Xin-yun 1,MAO Kan-lang1,2,PENG Chao-qiong1,WU De-sheng1,ZHOU Li 1,QIN Xiao-yun1,2,TAN Qin1,2 1.Shenzhen Center for Disease Control and Prevention,Shenzhen Key Laboratory of Modern Toxicology,Shenzhen 518055,China;2.School of Life Science,Shenzhen University,Shenzhen 518060,China;Role of CYP1A2 gene in trichlorethylene toxicity[J];职业卫生与应急救援;2013-03
8 SITU Jie,ZHANG Jian-jie,QIU Shao-hong,DENG Li-hua,YANG Xin-yue,ZHU De-xiang,GUO Mei-qiong Shenzhen Prevention and Treatment Center for Occupational Diseases,Shenzhen 518001,China;Epidemiological and clinical features of occupational medicamentosa-like dermatitis induced by trichloroethylene in Shenzhen from 2006 to 2011[J];职业卫生与应急救援;2013-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 SITU Jie,ZHANG Jian-jie,QIU Shao-hong,DENG Li-hua,YANG Xin-yue,ZHU De-xiang,GUO Mei-qiong Shenzhen Prevention and Treatment Center for Occupational Diseases,Shenzhen 518001,China;Epidemiological and clinical features of occupational medicamentosa-like dermatitis induced by trichloroethylene in Shenzhen from 2006 to 2011[J];职业卫生与应急救援;2013-03
2 HUA Ming,ZHENG Qian-ling,CAO Dan-yan,HE Zhen-feng,ZHANG Bi-zhu,ZHENG Gang,HUANG Yong-shun(Institute of Occupational Health Surveillance,Guangdong Province Hospital for Occupational Diseases Prevention and Treatment;Guangdong Provincial Key Laboratory of Occupational Diseases Prevention and Treatment,Guangzhou 510300,China);Investigation and analysis on nosocomial infection and drug resistance in occupational medicamentosa-like dermatitis due to trichloroethylene[J];中国职业医学;2013-03
3 ZENG Zi-fang,XIA Li-hua,WU Qi-feng,HUANG Jia-wen,CAI Pin,CHEN Ying(Guangdong Province Hospital for Occupational Diseases Prevention and Treatment;Guangdong Provincial Key Laboratory of Occupational Diseases Prevention and Treatment,Guangzhou 510300,China);Clinical observation on nutrition support therapy in patients with severe occupational medicamentosa-like dermatitis due to trichloroethylene[J];中国职业医学;2013-03
4 LI Zhi-min,ZHU De-xiang,GUO Mei-qiong Shenzhen Prevention and Treatment Center for Occupational Diseases,Shenzhen 518001,China;Analysis of reporting data of occupational diseases in Shenzhen during year of 2006~2010[J];职业卫生与应急救援;2013-01
5 ZHANG Jia-xiang1,ZHA Wan-sheng1,WANG Feng1,JIANG Tao1,XV Shu-hai1,SHEN Tong2,ZHU Qi-xing1,2;Changes of complements C3 and C4b expression in trichloroethylene-sensitized BALB / c mice[J];中国工业医学杂志;2013-01
6 Yuan Yefei,Hu Xiangyu,Ou Xianhong1 Department of Medicinal Chemistry;1Laboratory of Myocardioelectrophysiology,Luzhou Medical College;Effect of Chinense Pursh Penthorum on cells of alcoholic fatty liver induced by ethanol in vitro[J];泸州医学院学报;2013-01
7 JIANG Tao,XU Shu-hai,YU Jun-feng,SHEN Tong,ZHU Qi-xing(Department of Occupational and Environmental Health,School of Public Health,Anhui Medical University,Hefei 230032,China);Expression of TGF-β and IL-10 in sera and skin of TCE-sensitized guinea pig[J];中国职业医学;2012-01
8 LIU Qing,TENG Yan-xia,YI Juan,ZANG Dan,ZHOU Wei,DONG Hai-yan,NIU Yong,DUAN Hua-wei,HUANG Xian-qing,BIN Ping,ZHENG Yu-xin,DAI Yu-fei (National Institute for Occupational Health and Poison Control,Chinese Center for Disease Control and Prevention,Beijing 100088,China);Study on the expressions of Fas and Fas ligand in patients with dermatitis medicamentosa-like of trichloroethylene[J];中国职业医学;2011-05
9 LU Ye-chun1,LIU Yi-xiang1,WU Wei2,ZHOU Feng1,JI Bao-ping1,SU Chun-yuan1(1.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China; 2.College of Engineering,China Agricultural University,Beijing 100083,China);Preventive Effect of Blueberry Polyphenols on Oleic Acid-induced Fat Accumulation in HepG2 Cells[J];食品科学;2011-17
10 Chun-Hui Wang,Du-Xin Qing,Department of Gastroenterology,the Second Xiangya Hospital of Central South University,Changsha 410011,Hunan Province,China;Effect of palmitic acid on ethanol-induced fatty degeneration in hepatic cells in vitro[J];世界华人消化杂志;2011-19
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved