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《Journal of Clinical Medicine in Practice》 2014-05
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Expression and significance of decorin in Ishikawa cells

CAI Xiaojun;CHEN Ping;CAO Fengjun;LI Linjun;LI Fang;DENG Shouheng;Oncology Center,Shiyan People's Hospital;  
Objective To observe the effect of epidermal growth factor receptor( EGFR) signal transduction inhibitor RG-14260( RG) alone or combined with mTOR signal transduction inhibitor rapamycin( RA) on the proliferation and expression of decorin( DCN) in Ishikawa cells in vitro.Methods After treated with RG alone or combined with RA for 24 hours,immunohistochemical method was used to detect the expression of proliferating cell nuclear antigens( PCNA). Reverse transcriptase-polymerase chain reaction( RT-PCR) and Western blot method were used to detect the expression changes of DCN genes and proteins in cells respectively. Results RG alone or combined with RA were able to decrease the expression rate of positive PCNA cells in Ishikawa cells,promote the expression of DCN genes and proteins,but the efficacy of combined application was more significant.Conclusion Single and combined applications of EGFR and mTOR signal transduction inhibitors can up-regulate the expression of DCN in Ishikawa cells at the levels of genes and proteins,and meanwhile the increased DCN can reinforce the inhibitory effect of signal transduction inhibitors on the cell proliferation.
【Fund】: 湖北省教育厅基金(Q20092401);; 湖北医药学院基金(2008CXZ02);; 中国高校医学期刊临床专项资金(11321499)
【CateGory Index】: R737.33
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