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Study on tissue engineering skin for chemical irritation detection

ZHANG Guangjing;WANG Zhengxiang;FENG Shijun;AI Dongfang;Department of Dermatology,Cangzhou Central Hospital;  
Objective To undergo chemical irritation detection using a tissue engineering skin model established in vitro. Methods The tissue engineering skin model was constructed using bovine type Ⅰ collagen as scaffold and human normal skin keratinocytes and fibroblasts as cell sources. Twenty chemicals were selected as test substances from the substances specified by the European centre for validation of alternative experiments for skin irritation. During the detection,25 μl of 20 chemicals(25 mg solid were ground into powder before added into 25 μl distilled water) were contacted with the surface of the test model for 15 min. The cell viability was measured by MTT. Meanwhile, 25 μl distilled water was added to the negative control and 25 μl of 5 % sodium laurate was added to the positive control. Results Based on the relative cell viability values, 16 chemicals were identified by the reconstructed detection model. Conclusions The tissue engineering skin model determines the irritation of chemicals and can be used as a detection method for chemical irritation.
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