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《CHINESE PHARMACOLOGICAL BULLETIN》 1999-04
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Effects of gypenosides on c sis expression of bovine aortic endothelial cells and proliferation of vascular smooth muscle cells induced by lipopolysaccharide and active oxygen species

HUANG Hong Lin, CHEN Lin Xi, ZHU Bin Yang, YU Lin, LIAO Duan Fang (Division of Cardiopulmonary Pharmacology,Hengyang Medical College,Hengyang 421001)  
AIM To investigate the effects of gypenosides(Gyp) on c sis expression of endothelial cells (EC) and proliferation of vascular smooth muscle cells (VSMC)induced by lipopolysaccharide (LPS) and oxygen free radical(OFR) METHODS Cultured bovine aortic endothelial cells were incubated with Gyp (10~ 20 mg·L -1 )and LPS (16 mg·L -1 ) or xanthine(100 μmol·L -1 ) plus xanthine oxidase (100 U·L -1 ) C sis expression was determined by in situ hybridigation Nitrite, the matabolic product of NO, was measured according to a modified Griess method,and the proliferation of endothelial cells and VSMC was estimated by MTT assay. RESULTS We observed that LPS promoted c sis gene expression, and inhibited NO release, and that X XO inhibited EC proliferation and NO release Both LPS and X XO stimulated SMC proliferation by inducing growth factors release from EC Gyp significantly decreased c sis gene expression,increased NO synthesis and proliferation of EC Furthermore, Gyp indirectly inhibited SMC proliferation by blocking the effects of LPS and X XO. The effects of Gyp were abolished by NO synthase inhibitor N G L Arg CONCLUSION Gyp inhibited X XO and LPS induced proliferation of VSMC by attenuating c sis gene expression and promoting NO synthesis/release of EC.
【Fund】: 国家自然科学基金 湖南省教委资助
【CateGory Index】: R963
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【References】
Chinese Journal Full-text Database 3 Hits
1 Sun Wansen,Wu Xili,Qiao Chenglin The Second Hospital Attached to Xi'an Jiaotong University,Xi'an (710004);Clinical Study of Gypenodide on Therapeutic Chronic Renal Failure[J];Journal of Zhejiang College of Tcm;2004-02
2 Gao Xiaoge, Cui Wei (Tianjin First Central Hospital, Tianjin 300192);Studies on some Traditional Chinese Medicine with Efficacy of Protecting Vascular Endothelial Cell[J];Tianjin Pharmacy;2003-02
3 SHANG Gang wei 1, JANG Yong pei 1, MEI Qi bing 2, ZHAO De hua 2 (1. Xijing Hospital, Fourth Military Medical University; 2. Fourth Military Medical University, Xi'an Shanxi 710032, China);Key technology of the methodology of fingerprints-pharmacy of traditional Chinese medicines (F-P of TCMs)[J];Chinese Traditional Patent Medicine;2002-03
【Citations】
Chinese Journal Full-text Database 5 Hits
1 Lei Jianhua;et al(Department of Physiology Hengyang healthy academy,Hengyang,Hunan);Preventive Effect of Gypenoside Against Endotoxic Shock-Induced DIC in rabbit[J];JOURNAL OF HENGYANG MEDICAL COLLEGE;1994-02
2 HUANG Hong-Lin;YIN Wei-Dong;LIAO Duan-Fang;CHEN Jian-Xiong;TANG Xiao-Qing;LI Bo-Ping;CAO Jian-Guo and YU Lin (Division of Cardiopulmonary Pharmacology,Institute of Cardiovascular Diseases,Hengyang Medical College,Hengyang 421001,Chinas);Effect of Gypenosides on Formation of Atherosclerotic Plaque of Aorta in Hypercholesterolemic Rabbits[J];CHINESE JOURNAL OF ARTERIOSC LEROSIS;1998-04
3 LIAO DuanFang;YIN Wei-Dong;CHEN JianXiong;TEN Hua;TAN Xiao-Qing;LI Bo-Ping and YU Lin (Department of Cardiopulmonary Pharmacology,Institute of Cardiovascular Disease,Hengyang Medical College,Hengyang 421001,China);Effect of Gypenosides on Nitric Oxide and Lipid Peroxidation in Blood-serum of Diet-induced Atherosclerotic Rabbit[J];CHINESE JOURNAL OF ARTERIOSC LEROSIS;1994-04
4 IU Li-Bo,HU Bi,LIAO Duan-Fang1 (Dept of Physiology; 1Dept of Pharmacology, Hengyang Medical College,Hunan 421001);Protective effect of gypenosides on free radical damage of isolated guinea pig papillary muscles[J];Chinese Pharmacological Bulletin;1993-04
5 XIAO Guan-Lian,LIAO Duan-Fang; CHEN Jian-Xiong; YU Lin (Dept.of pharmacology, Hengyang Medical College, Hunan 421001);Protective effect of gypenosides on OFR-induced damage of relaxing capacity of rabbit thoracic aortae in vitro[J];CHINESE PHARMACOLOGICAL BULLETIN;1994-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Dong sheng, YUAN Zhao kai, HUANG Xian ping,XIE Meng zhou, LU Fang guo, YIN Wen xue(Post doctor Station of Hematology, Xiangya Hospital,Central South University, Hu′nan Changsha 410008, China);Relationship Between Syndrome Differentiation of Phlegm & Blood Stasis andPDGF-A mRNA Expression of Peripheral Blood Mononuclear Cell[J];Journal of Anhui Traditional Chinese Medical College;2003-02
2 MA Shan feng,LU Zong lan,GUAN Su dong,LIU Gui lan (Department of Physiology,Bengbu Medical College,Anhui 233003 ,China) [;Antagonistic effect of gypenoside on diaphragm against doxorubicin cytotoxicity[J];JOURNAL OF BENGBU MEDICINAE COLLEGE;2000-05
3 Wu Jianming Laboratory of Cardiopulmonary Endocrinology;THE EFFECT OF Mg~(2+) AND TAURINE ON ENDOTHELIN-STIMULATED MULTIPLICATION OF VASCULAR SMOOTH MUSCLE CELLS[J];;1991-05
4 Institute of Cardiovascular Research Zhou Ai ru,et al;THE PRIMARY REPORT OF P53 GENE MUTATION IN HUMAN ATHEROSCLEROSIS[J];JOURNAL OF BEIJING MEDICAL UNIVERSITY;1994-S1
5 Ji Yang; Zheng Guoqian; Sun Duxin; Lin Aiyou; Rui Yaocheng(Department of Pharmacology, College of Pharmacy, Shanghai,200433);Interleukin-1 Alpha Induced Proliferation of Bovine Cerebromicrovascular Smooth Muscle Cells and Antagonism of Drugs[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1994-01
6 Ji Yang; Zeng Guoqian; Rui Yaocheng (Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai, 200433);Inhibitory Effects of Imperatorin and Iso-imperatorin on Tumor Necrosis Factor Release from Mouse Peritoneal Macrophages[J];Academic Journal of Second Military Medical University;1994-06
7 Wang Mei, Wang Xukai, Liu Guangyao (Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, 400042);Effects of insulin on proliferation of human vascular smooth muscle cells[J];ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE;1998-01
8 HOU Xiao-Ping; JIA Guo-Liang; ZhAOKuan; JIA Guang-Xing;; DA Ji-Ping(Department of Cardiovasology, Xijing Hospital,Fourth Military Medical University, Xi'an 710033)(General Hospital of Air Force,PLA);Effects of gypenosides on intimal proliferation and expression of c-sis oncogene in rabbit iliac artery afterballoon deendothelialization[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1998-05
9 Wang Rui, Li Ying,Shuxin,Zhang Lili,Jia BinDepartment of Pathophysiology , Xi’an 710033;Effects of adrenoceptor activators and blockers on the proliferationand c-fos,C-myc oncogene expression of vascular smooth musclecells[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1995-01
10 ZENG Rong, LIU Xiao-rong, LI Jin, DAI Yun, AN Jin (Department of Histology and Embryology, First Military Medical University, Guangzhou 510515, China);Biological effect of transient expression of 3 antisense c-myc RNA on the vascular smooth muscle cells in vitro[J];Journal of First Military Medical University;2000-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Chen Qianfen;Tian Hecun;Zhang Chenyin;Chu Dekai;Yang Weidong(Department of Pathophysilolgy,Bengbu Medical College,Anhui 233003);Protective effect of gypenosides on global cerebral ischemia-reperfusion injury in rat[J];ACTA ACADEMIAE MEDICINAE BENGBU;1996-06
2 Liu Yuanyuan, et al(Department of Infectious Diseases);EFFECT OF PANAXADIOL SAPONIN ON LIPlDPEROXIDE OF TISSUES IN RATS WITH ENDOTOXIC SHOCK[J];;1990-04
3 Liu Yuanyuan, Lu Meide, Zhao Xuejian Wang Zhongshan, Zhao Dan(Dept. of Infect. Dis, First Teaching Hosp., Dept. of Pathophysiology);EFFECT OF PANAXADIOL SAPONIN ON 5-HT IN RATS WITH ENDOTOXIC SHOCK[J];;1991-04
4 SONG Yan 1,SONG Wen zhe 1,TAN Yan 2* (1. Dept.of General Surgery, China Japan Union Hospital, Jilin University, Changchun 130031, China;2. Dept.of Central Laboratory,The First Hospital,Jilin University) [;Experimental Study on the Protective Effect of Aminoguanidine on Liver Injury in Rodent Model of Endotoxic Shock[J];Journal of Jilin University of (Medicine Edition);2003-05
5 Wang Yahong ,Guo Weiqin,Wang Shuoren,et al (Dongzhimen Hospital Affiliated to Beijing University of TCM);Experimental Research on Effects of Jiangzhitongmaifang's Pharmacological Serum on Vascular Endothelial Cells[J];China Journal of Traditional Chinese Medicine and Pharmacy;2004-10
6 Niu Dadi,et al Laboratory of Cardiopulmonary Endocrinology;TAURINE INHIBITS VASCULAR SMOOTH MUSCLE CELL PROLIFERATION OF RAT[J];;1993-04
7 GF Shujun;TANG Chaoshu ;PANG Yongzheng ;CHENG Shi(Department of Biophysics,Beijing Medical University100083);PROTECTIVE EFFECT OF METALLOTHIONEIN ON RAT LIVER MITOCHONDRIA DAMAGED BY OXYGEN FREE RADICAL[J];Journal of Beijing Medical University;1996-06
8 WAN De guang, PEI Jing (万德光,等) Pharmacy College, Chengdu University of TCM(Chengdu,610075,China);The Position and Function of Herbal Species Appraisement in Quality Control of Traditional Chinese Medicine[J];JOURNAL OF CHENGDU UNVERSITY OF TARDITIONAL CHINESE MEDICINE;2000-03
9 Chu Zhiyong, Gong Chungui, Rui Yaocheng (Department of Pharmacology,College of Pharmacy,Second Military Medical University, Shanghai, 200433);Inhibitory effects of imperatorin on TNF induced adhesion reaction of BCMEC with monocytes[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1998-02
10 LI Li,PENG Jian-ying~*,BAI Rui-xia,HAN Bin(College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071001,China);SRAP and TRAP Markers and their Application to Research about Horticultural Plants[J];Acta Botanica Boreali-Occidentalia Sinica;2006-08
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 CHU Xiu-ling et al (College of Agronomy,Liaocheng University,Liaocheng,Shandong 252000);Research Progress in the Antibacterial of Chinese Traditional Medicine and its Clinical Aapplication[J];Journal of Anhui Agricultural Sciences;2008-21
2 HOU Hui-li~1,WANG Bao-li~1,GUO Yun-cheng~2,MAO Jianxiang~2,FU Tong-sheng~1(College of Animal Medicine,HNAU,Changsha,Hunan,410128,China;2.Taojiang Institute of Gynostemma Pentaphyllum,Yiyang,Hunan,413517,China);Effect of "Nanshen Xubao" on Activities of Serum Enzymes and Lactate Dehydrogenase Isozyme-Spectra in Finishing Pigs[J];Progress in Veterinary Medicine;2006-07
3 HOU Hui-li,FU Tong-sheng(College of Animal Medicine,Hunan Agricultural University,Changsha,Hunan,410128,China);Research Advance in the Chemical Ingredients and Pharmacological Effects of Gynostemma pentaphyllum[J];Progress in Veterinary Medicine;2006-S1
4 TAN Huabing,LU Side,ZHONG Xiaodong.People's Hospital of Yunyang Medical College,Shiyan 442000,China;Research of interference effect of Fiveleaf Gynostemma Herb on hyperlipomia and C-reaction protein rise in the rabbits caused by rich hypercholesterolemia diet[J];Journal of Practical Diagnosis and Therapy;2006-11
5 ZHANG Yong,DING Guohua,ZHANG Jiane,et alDivision of Nephrology,The Affiliated Renmin Hospital of Wuhan University,Wuhan (430060);Effect of Gypenosides on Expression of Connective Tissue Growth Factor(CTGF) in UUO Rats with Renal Tubulointerstitial Fibrosis[J];Chinese Journal of Integrated Traditional and Western Nephrology;2005-07
6 ZENG Xian-hui,WEI Qing,ZHOU Shu-ning(Department of Heart Medicine,The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250001,Shandong,China);Recanalization after Myocardial Coronary Microcirculation of Progress in Chinese Medicine Research[J];Liaoning Journal of Traditional Chinese Medicine;2009-02
7 LUO Wen-zheng1,ZHANG Qing-zhong2,LV Zhi-ping1(1.Southern Medical University,Guangzhou 510515,Guangdong,China;2.Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong,China);The Application of Gynostemma Pentaphyllum Makino in Fatty Liver Disease Treatment[J];Journal of Liaoning University of Traditional Chinese Medicine;2009-08
8 TONG Yuqing(The College of Mongolian Medicine of Inner Mongolian University for Nationalities);Modernization Research and Information Technology of Prescription of Mongolian Medicine[J];Chinese Journal of Ethnomedicine and Ethnopharmacy;2006-02
9 Lin Qingqiang,Lin Yu,Zhu Peng(College of Life Science,Fujian Normal University,Fuzhou,Fujian 350108,China);Gynostemma Role in the Health and Beverage Manufacturing Inquiry[J];Academic Periodical of Farm Products Processing;2009-01
10 WANG Xu-ping,ZHAO Ling,FENG Yu-xin,SHANG Lin-shan,LIU Jin-cheng,CAO Wei-peng,ZHU Xiao-yin,XIN Hua(Institute of Cell Biology,School of Medicine,Shandong University,Jinan 250012,Shandong,China);Protection of gypenoside on glutamate-induced oxidation in the cultured cortical neurons of embryonic rats[J];Journal of Shandong University(Health Sciences);2006-06
China Proceedings of conference Full-text Database 1 Hits
1 Huang Ping Qian kang;Analysis of Gypenosides on early Diabetic Nephropathy by Westen blot[A];[C];2006
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Lei Jianhua;et al(Department of Physiology Hengyang healthy academy,Hengyang,Hunan);Preventive Effect of Gypenoside Against Endotoxic Shock-Induced DIC in rabbit[J];JOURNAL OF HENGYANG MEDICAL COLLEGE;1994-02
2 JIAO Hong-Li;;LIU Yi-Lin;; WAN La-Xiang;; HU Long-Mei;;YANG He-Ping and YANG Yong-Zong(Molecular Biology Center,Hengyang Medical College,Hengyang 421001, Guangdong Province Yuebei Hospital,Shaoguan 512026, Nanyue Pharmaceutical Factory,Hengyang 421001. C;Effects of L-arginine on Atherosclerotic Plaque Area of Aortae and Coronary Arteries in Rabbit with Hypercholesterolemia[J];CHINESE JOURNAL OF ARTERIOSC LEROSIS;1997-03
3 LIAO DuanFang;YIN Wei-Dong;CHEN JianXiong;TEN Hua;TAN Xiao-Qing;LI Bo-Ping and YU Lin (Department of Cardiopulmonary Pharmacology,Institute of Cardiovascular Disease,Hengyang Medical College,Hengyang 421001,China);Effect of Gypenosides on Nitric Oxide and Lipid Peroxidation in Blood-serum of Diet-induced Atherosclerotic Rabbit[J];CHINESE JOURNAL OF ARTERIOSC LEROSIS;1994-04
4 Xin Dongsheng;Li Donghui Department of Pharmacology;STUDY OF EFFECTS OF GYPENOSIDES ON ANTI-MYOCARDIAL ISCHEMIA[J];Journal of Xi'an Jiaotong University (Medical Sciences);1991-02
5 Chen Chao , Fang Dachao ( Dept. of Pharmacology, Tongji Medical University, Wuhan 430030 )Department of Pharmacology,Yichang Medical College,Hubei 443003;EFFECTS OF PALMATINE ON ELECTROPHYSIOLOGICAL CHARACTER IN THE ISOLATED GUINEA PIG PAPILLARY MUSCLES[J];Chinese Pharmacological Bulletin;1991-02
6 Li Lin1, Xing Shantian, Zhou Jinhuang ( Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850);PROTECTIVE EFFECTS OF GYPENOSIDES ON RAT HEPATIC LIPID PEROXIDATION AND MEMBRANE FLUIDITY DAMAGE: IN VITRO STUDIES[J];Chinese Pharmacological Bulletin;1991-05
7 IU Li-Bo,HU Bi,LIAO Duan-Fang1 (Dept of Physiology; 1Dept of Pharmacology, Hengyang Medical College,Hunan 421001);Protective effect of gypenosides on free radical damage of isolated guinea pig papillary muscles[J];Chinese Pharmacological Bulletin;1993-04
8 XIAO Guan-Lian,LIAO Duan-Fang; CHEN Jian-Xiong; YU Lin (Dept.of pharmacology, Hengyang Medical College, Hunan 421001);Protective effect of gypenosides on OFR-induced damage of relaxing capacity of rabbit thoracic aortae in vitro[J];CHINESE PHARMACOLOGICAL BULLETIN;1994-02
9 ZHAO Ying, HU DaYi(Lab of Heart Center, Beijing Red Cross Chao Yang HospitalAffiliate of Capital University of Medicine, Beijing 100020);Effect of GP on action potentials,Lcalcium channel currents, sodium channel currents and potassium channel currents in isolated single ventricular myocyte of guinea pigs[J];CHINESE PHARMACOLOGICAL BULLETIN;1998-01
10 CHEN Li-Feng,QIU Sai-Hong,LI Qun-Ai,HE Yong-Jian (Hunan Academy of Traditional Chinese Medicine and Materia Medico,Changsha 410006);Comparison between the effcts of gypenosides and ginsenosides on cardiac function and hemodynamics in dogs[J];Chinese Journal of Pharmacology and Toxicology;1990-01
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