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Effect of ganoderma polysacchrides on PKC activity in murine peritoneal macrophages

LI MingChun, LEI LinSheng1, LIANG DongSheng, XU ZiMing, YANG ShuQin1, SUN LiSha1 (Dept of Pharmacy, 401 Hospital of PLA, Qingdao 266071)  
AIM To investigate the effect of Ganoderma polysacchride(GLB 7) on PKC activity in murine peritoneal macrophages METHODS A new ionpair reversedphase high liquid chromatography method was used to determinate the activity of protein kinase C in cellsRESULTS GLB 7 (40 mg·L -1 )induced activation,translocation of PKC, and GLB 7 antagonized the inhibition effect to PKC of staurosporine(10 μmol·L -1 ) in murine peritoneal macrophages The peak time was 30 minutes,the activity of PKC came back the basic level at 2 hours CONCLUSION The immunopoteniating effect of Ganderma polysacchride may be associated with its activation of PKC in murine peritoneal macrophages
【Fund】: 国家自然科学基金资助项目!No39400165
【CateGory Index】: R285
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