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Changes of phospholipase D activity of exudate leukocytes in rat carrageenan-induced pleurisy

LI Ping, LU YunBi, ZHOU HanLiang, CHEN JiQiang (Dept of Pharmacology, Medical School, Zhejiang University, Hangzhou 310031)  
AIM To observe the relationship between the changes of phospholipase D(PLD) activity in inflammatory leukocytes and inflammation in vivo METHOD Inflammation was carried out by the carrageenaninduced pleurisy in the rat, and the exudate volume, total account of influxed cells and total activity of myeloperoxidase in exudate which was the index of degranulation of neutrophils were measured as the severity of inflammation PLD activity was assessed by the enzymecoupled spectrophotometric assay RESULT PLD activity of influxed leukocytes increased 40~60 times than that of peripheral blood leukocytes taken from untreated animals which contain low levels of PLD activity 〔(014±003) μmol·g -1 ·min -1 〕, and the influxed cellassociated PLD activity peaked at 3 h after injection of the carrageenan However,the severity of inflammation peaked at 12 h after carrageenan injection 500 μg and 1000 μg carrageenan caused dosedependent exudation in the pleural cavity but no significant difference of PLD activity was observed at 12 h after injection of carrageenan Indomethacin(2 mg·kg -1 , ip) and dexamethasone(01 mg·kg -1 , ip) both significantly attenuated the pleural exudation and cellular influx at 6 h after injection of carrageenan but had no significant effects on influxed cellassociated PLD activity CONCLUTION These findings indicate that the PLD activity of inflammatory cells in rat carrageenaninduced pleurisy increased, and suggest that this increase of PLD activity is not a secondary but a primary effect in the present model of acute inflammation, and antiinflammatory mechanisms of low dose of indomethacin and dexamethasone are not linked to PLD
【Fund】: 国家自然科学基金资助课题!No39670836
【CateGory Index】: R96
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