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《CHINESE PHARMACOLOGICAL BULLETIN》 2000-04
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Antagonistic effect of metallothionein against homocysteine induced vascular endothelial cell injury

QI Yong Fen, LI Shu Lian, WANG Xiao Hong, Chen Ya Hong, PANG Yong Zheng, CHEN Qing, TANG Chao Shu (Institute of Cardiovascular Research,the First Hospital,Beijing Medical University,Beijng 100034)  
AIM To observe the antagonistic effect of metallothionein (MT) against homocysteine (Hcy) induced endothelial cell injury. METHODS Human vascular endothelial cells (VECs) were cultured. LDH leakage of VECs and content of MT in VECs were measured. The viabilities of VECs were calculated by trypan blue exclusion. RESULTS Hcy not only increased LDH leakage of cultured VECs but also decreased the viabilities of cultured VECs in dose dependent manner. Exogenous MT (5×10 -8 mol·L -1 ) could inhibit Hcy induced VECs injury in dose dependent manner. LDH leakage in MT(5×10 -8 mol·L -1 ) plus Hcy (1 0 mmol·L -1 ) group decreased by 27 1%( P 0 05), whereas the viabilities of VECs increased by 6% ( P 0 05), compared with Hcy group. MT production of cultured VECs was induced by preincubation of thrombin. MT content (in 1 000 U·L -1 thrombin induced cells) increased by 32 5%( P 0 01) compared with control. LDH leakage of cultured VECs in thrombin plus Hcy group decreased by 46%( P 0 01), and the viabilities of cultured VECs increased by 8% ( P 0 05), compared with Hcy alone group. CONCLUSION Whether administration of exogenous MT or induction of endogenous MT formation can diminish Hcy induced VECs injury.
【Fund】: 国家自然科学基金资助课题 !No 39730 2 2 0
【CateGory Index】: R96
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