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《Chinese Pharmacological Bulletin》 2003-11
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Protective effects of metallothionein induced by Zn~(2+) on ischemia/reperfusion myocardium in isolated rat heart

KE Qin-Mei, FENG Yi-Bai 1, CHENG Bei,GUAN Si-Ming, LI Wei, CHEN Xue-Lin (Dept of Geriatrics, 1Institute of Cardiology, the Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022)  
AIM To investigate the protective effects of metallothionein induced by Zn 2+ on ischemia/reperfusion myocardium in isolated rat heart. METHODS 32 Sprague-Dawley rats were randomly divided into 4 groups (n=8, respectively): control group, ischemia/reperfusion (I/R) group, Zn 2+ pretreated group (ZPC), PD98059 (inhibitor of extracellular signal regulated protein kinase)+Zn 2+ pretreated group (PZPC). The levels of creatime kinase (CK), lacatate dehydrogenase (LDH) and adenosine triphosphate (ATP), which indicated myocardium injury, and the cardiac function(LVSP and±dp/dt max), were observed in this study. The expression levels of MT were valued by the 109Cd/hemoglobin affinity assays. RESULTS The expression level of MT was higher in ZPC group than in control group and I/R group. Compared with I/R group, the level of LDH and CK was reduced, ATP content was increased, and cardiac function (LVSP and±dp/dt max) was improved (P0.01) in ZPC group. The correspond data between ZPC group and control group had no significant difference. While the protective effects of ZPC were inhibited by PD98059. CONCLUSION High expression of MT induced by Zn 2+ has protective effects on ischemia/reperfusion myocardium in isolated rat hearts. This may olne to activity of mitogen activated protein kinase pathway.
【CateGory Index】: R96
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