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《Chinese Pharmacological Bulletin》 2004-12
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The effects of ganoderma lucidum polysaccharides peptide (GLPP) on the nitric oxide production in mice peritoneal macrophages

YOU Yu-hong, LIN Zhi-bin (Dept of Pharmacology, School of Basic Medical Science,Peking University Health Science Center,Beijing 100083,China)  
Aim To study the effects of Ganoderma polysaccharide peptide (GLPP) on the production of nitric oxide (NO) in mice peritoneal macrophages induced by LPS and its mechanism. Methods The effects of GLPP on the NO releasing from mice peritoneal macrophages induced by LPS were detected by using Griess reagent and the expression of iNOS by GLPP in mice peritoneal macrophages was detected by immunohistochemical method.Result The production of NO was increased by GLPP.(25~200 mg·kg -1) ig for five days or by GLPP(3.125~200 mg·L -1) in vitro but the effects of LPS on the production of NO was not influenced significantly. The expression of iNOS was increased by GLPP(25~200 mg·kg -1) ig for five days or GLPP(3.125~200 mg·L -1) in vitro.Conclusion GPP in vivo or in vitro could increase the production of NO in mice peritoneal macrophages. It might take effects by enhancing the synthesis of iNOS.
【Fund】: 上海绿谷 (集团 )有限公司科研基金资助项目
【CateGory Index】: R285
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