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《Chinese Pharmacological Bulletin》 2005-02
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Effects of sini decoction on intestinal mucosa cell apoptosis and its ceramide mechanism after intestinal ischemia reperfusion in rats

LIU Ke-xuan1,WU Wei-kang1,ZHU You-kai3,LIU Chui-liang4,SUN Hui-lan 2,ZHAO Ming-qi2 (1.Dept of Anesthesiology,the First Affiliated Hospital, 2.Institute of Integr ated Tradition Chinese and Western Medicine; 3.Dept of Pathology,Basic Medical College of Sun Yat-sen University,Guangzhou 5 10080,China; 4.Dept of Anesthesiology,the 2nd Affilliated Hospital of Guangzhou Tradition Med icine University,Guangzhou 510120,China)  
Aim To investigate the effects of sini decoction(SND) on intestinal mucosa cell apoptosis and its mechanism after intestinal ischemia reperfusion in rats.Methods Twenty-four Sprague-Dawley rats of both sexes were randomly divided into 3 equal groups:①control group in which sham operation was performed;②model group in which intestinal I/R was produced by clamping super mesenteric artery(SMA) for 1 hour and declamping SMA for 3 hours;③SND group in which SND(3 g·kg -1)was given via stomach tube for 3 days before operation. A strip of small intestine was taken from distal end of ileum for electron microscopic examination; SOD activity, MDA and ceramide concentration of intestinal mucosa were detected;Apoptosis of intestinal mucosa cell was examined by TUNEL method and Sphingomyelinase (SMase) mRNA expression was analyzed by RT-PCR.Results In model group, many typical apoptotic epithelial cells were seen under electron microscopic, apoptosis index was higher than that in control group(P0.01). SOD activity of model group was significantly lower than that of control group(P0.01), but MDA and ceramide content as well as SMase mRNA expression were obviously higher than those in control group(P0.01).In addition, In model group, ceramide content was positively correlated to apoptosis index(r=0.852,P0.01),and negativel y to SOD activity (r=-0.775,P 0.01).The pretreatment of SND change d the above abnormal phenomena significantly(P0.01). Conclusion SND can reduce intestinal mucosa cell apoptosis by removing oxygen free r adical, downregulation of SMase mRNA expression and suppression of ceramide production.
【Fund】: 广东省中医药管理局基金资助项目(No1040066)
【CateGory Index】: R285
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