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《Chinese Pharmacological Bulletin》 2005-03
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Effects of losartan on the expression of STAT1 in glomeruli of diabetic rats

SHI Yong-hong, DUAN Hui-jun, WANG Li-hui,HE-Ning, LIU Qing-juan (Dept of Pathology,Hebei Medical University, Shijiazhuang,050017,China)  
Aim To investigate the effects of losartan on activation of signal transducers and activators of transcription 1(STAT1) protein and the expression of STAT1 mRNA in glumeruli of diabetic rats.Methods Wistar male rats were randomly divided into three groups: control group, diabetes group and treatment group.Diabetes was induced by intraperitoneal injection of STZ (65 mg·kg~-1 ). Losartan (40 mg·kg~-1 ) was administered daily by gavage from the next day of the induction to diabetes for 2 weeks. The protein expression of p-STAT1 in glomeruli was observed by immunohistochemistry and western blot. STAT1 mRNA was measured by reverse transcription and polymerase chain reaction (RT-PCR).Results Compared with the control group rats, the expression of p-STAT1 was significantly increased in glomeruli in the diabetic rats (P0.01). After treatment with losartan, the expression of p-STAT1 was decreased (P0.05). The expression of STAT1 mRNA was significantly elevated in glomeruli in diabetic group rats. However losartan had no effect on the expression of STAT1 mRNA.Conclusion STAT1 signal pathway may be involved in the kidney damage associated with diabetes.Regulation of phosphorylation of STAT1 may be responsible for the renal protective effects of losartan in diabetic rats.
【Fund】: 河北省自然科学基金资助项目(NoC2004000536)
【CateGory Index】: R96
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