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《Chinese Pharmacological Bulletin》 2006-02
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Effects of valsartan on the expression of STAT1 and STAT3 in glomerular mesangial cells under high concentration of glucose

SHI Yong-hong,DUAN Hui-jun,WANG Li-hui,REN Yun-zhuo,GAO Feng(Dept of Pathology,Hebei Medical University,Shijiazhuang 050017,China)  
Aim To investigate the effects of valsartan on activation of signal transducers and activators of transcription 1,3 in glomerular mesangial cells(GMCs) under high concentration of glucose.Methods we used high concentration glucose and valsartan to stimulate the cultured rat GMCs in vitro.The protein expressions of signal transducer and activatior of transcription 1,3(STAT1,STAT3),p-STAT1 and p-STAT3 were observed by Western blot.The protein synthesis of TGF-β_1,fibronectin and type IV collagen in the supernatants of the GMCs were detected by enzyme-linked immunoadsorbent assay(ELISA) and radioimmunoassay.TGF-β_1 mRNA was measured by reverse transcription and polymerase chain reaction(RT-PCR).Results Compared with low glucose control group,the expressions of p-STAT1,p-STAT3 and TGFβ_1 mRNA were significantly increased in GMCs under high concentration glucose medium and there observed the high concentration of TGF-β_1,fibronectin and type IV collagen in the supernatants.The expression levels of p-STAT1,p-STAT3 and TGF-β_1 mRNA were significantly lower in the valsartan group than in those the high concentration glucose group.The concentration of TGF-β_1,fibronectin and type IV collagen in the supernatants in the valsartan group were lower than that in the high concentration glucose control group.Conclusion Valsartan can inhibit overproduction of TGF-β_1 and ECM proteins in GMCs under high concentration of glucose,partly by regulating the phosphorylation of STAT1 and STAT3.
【Fund】: 河北省自然科学基金资助项目(NoC2004000536)
【CateGory Index】: R965
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