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The effects of glycosides of cistanche on learning and memory in β-amyloid peptide induced Alzheimers disease in mice and its possible mechanism

LIU Feng-xia,WANG Xiao-wen,LUO Lan,XIN Hua.NA Bi,WANG Xue-fei(Dept of Pharmacology,Xin jiang Medical University,Wulumuqi 830054,China)  
Aim To study the protective effects of gly-cosides cistanche(GCs) on model of Alzheimers dis-ease(AD) in mice induced by β-amyloid peptide(β-AP) and its mechanism.Methods AD animal model was established by a single intracerebroventricular injection of micromolar doses of β-AP_(25-35) in mice.After 10 days,a step-through type passive avoidance training was performed on the mice for one time,followed by a ability assessment of learning and memory;superoxidase dismutase(SOD) activities,gultathionine peroxides(GSH-Px) activities and malondialdehyde(MDA) contents in mice brain were also assessed;the pathological changes in the AD mice brain were observed by electronic microscopy;TUNEL method was used to observe the apoptosis of cells;the immunohistochemical SABC method was used to determine expression of Bcl-2 and Bax.Results GCs markedly enhanced the ability of learning and memory which induce by β-AP in AD mice and increased the activity of SOD and GSH-Px,and reduced MDA contents in mice brain;GCs also ameliorated some pathological features of AD mice brain and significantly decreased cell apoptosis in mice brain.A decrease in the Bax expression and a increase in the Bcl-2 expression were also observed.Conclusions GCs significantly improves the ability of learning and memory induced by β-AP in AD mice and its possible mechanism of action may involve: Enhancement of free radical scavengers;inhibition of lipid peroxidation;inhibition of β-AP precipitation and apoptosis in mice brain cells induced by β-AP.
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