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《Chinese Pharmacological Bulletin》 2007-07
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Influence of PSS on glomerular capillary basement membrane in rat model of type 2 diabetes

QI He1,2,LI Zhi1,ZHAO Mei-mi1,YU Li-feng1, DUAN Ya-le1 (1.Dept of naturally occurring drugs, China Medical University, Shenyang 110001, China; 2. Dean′office of Technological College,Liaoning University of Traditional Chinese Medicine, Shenyang 110101,China)  
Aim To observe effects of PSS(polysaccharide)on glomerular capillary basement membrane(GBM) in type 2 diabetic rats. Methods Type 2 diabetic animal model was established by high-sugar and high-fat diets, associating injection of small count streptozotocin(STZ 20 mg·kg-1,iv). Adult male wistar rats were divided into four groups: normal control rats, non-treated diabetic rats and diabetic ratstreated with PSS ( 180 mg·kg-1·d-1), diabetic rats treated with Lovastatin. Plasma glucose, Creatinine clearance rate(Ccr), 24 h UAER, and profile of kidney hypertrophy were observed after 8 weeks. Protein expressions of TGF-β1 and CⅣ in renal were measured by immunohistochemistry. Renal tissues were analyzed by electron microscope. Results After 8 weeks, levels of UAER, profile of kidney hypertrophy and the thickness of GBM were obviously increased in diabetic rats. TGF-β1 and CⅣ were obviously higher expressed in diabetic rats than normal rats. In PSS-treated group, blood glucose level was decreased slightly but significantly. Expressions of TGF-β1, CⅣ were significantly decreased in PSS-treated diabetic rats than those untreated (P0.01). UAER, the thickness of GBM was significantly decreased in PSS-treated diabetic rats than that untreated (P0.05). But Ccr and profile of kidney hypertrophy showed no significant differences between the diabetic group and PSS-treated group. Conclusion PSS has a favorable effect on renal function and morphology in diabetic rats, which may be due to decreasing blood glucose, expressions of TGF-β1 and CⅣ and inhibiting the thickness of GBM, and then protecting glomerular filtration barrier.
【CateGory Index】: R587.2
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