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《Chinese Pharmacological Bulletin》 2007-09
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Effects of chlorzoxazone on HepG2 tumor cells

FAN Chang-long1,LUAN Yan1,CHEN Yan1,ZHANG Jun1,ZHANG Wan1,YANG Bao-feng1,2,DONG De-li1,2(1.Dept of Pharmacology,Harbin Medical University,2.Bio-pharmaceutical Key Laboratory of Heilongjiang Province,Harbin 150086,China)  
Aim To investigate effects of chlorzoxazone on survival and apoptosis of HepG2 cells.Methods The necrosis of HepG2 cells was evaluated by measurement of LDH release.The effects of chlorzoxazone on survival of HepG2 cells were assayed by MTT dyereduction.The effects of chlorzoxazone on cell apoptosis was analyzed by TUNEL method.The ultrastructure of HepG2 cells was observed by transmission electron microscope.Results Chlorzoxazone at concentrations of 100~500 μmol·L-1 inhibited survival ratios of HepG2 cells in a dose-dependent manner significantly.Typical apoptotic changes were observed in HepG2 cells under the fluorescence microscope and transmission electron microscope.Apoptosis of HepG2 cells was induced after treatment of chlorzoxazone at concentrations from 100 μmol·L-1 to 500 μmol·L-1 for 48h,which showed obvious concentration-effect relationship.The apoptotic ratios of HepG2 cells were also increased when chlorzoxazone(100,200,300 and 500 μmol·L-1) was treated for 24,48 and 72 h,which showed obvious time-effect relationship.Conclusion Chlorzoxazone inhibited HepG2 cells survival and induced cell apoptosis.
【Fund】: 黑龙江省杰出青年基金;; 教育部“新世纪优秀人才支持计划”资助
【CateGory Index】: R96
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