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《Chinese Pharmacological Bulletin》 2008-03
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Protective action of total glucosides of paeony on renal tubulointerstitium and its mechanism in diabetic rats

FANG Fang, WU Yong-gui, DONG Jing,REN Ke-jun, QI Xiang-ming, LIANG Chao, ZHANG Wei (Dept of Nephrology, the First Affiliated Hospital,Anhui Medical University,Hefei 230022,China)  
Aim To study the protective action of total glucosides of paeony(TGP)on renal tubulointerstitium and its mechanism in diabetic rats.Methods Diabetes was induced by injection of streptozotocin in rats.Rats was randomly divided into three groups: control group, model group, model group treated with TGP.TGP (50, 100, 200 mg·kg-1·d-1) was orally administered once a day for 8 wk.Tubulointerstitial morphological analysis was performed in PAS stained section.Expressions of osteopontin (OPN) and α-smooth muscle actin (α-SMA) in renal tubulointerstitium were determined by immunohistochemistry method, expression of nitrotyrosine (NT) was measured by Western blot analysis.Results Increased indices for tubulointerstitial injury were significantly ameliorated by TGP treatment with 100 and 200 mg·kg-1 in diabetic rats (P0.05,0.01).Elevated expression of OPN protein in renal tubulointerstitium in diabetic rats was only significantly inhibited by TGP treatment with 100 and 200 mg·kg-1(P0.01), and increased expression of α-SMA protein was significantly inhibited by TGP treatment with 50, 100 and 200 mg·kg-1(P0.01).Western blot analysis noted that the expression of NT protein was increased 3.4 fold in the kidney in diabetic rats,TGP treatment with 50, 100 and 200 mg·kg-1 could reduced the increased expression of NT protein by 41.2%、43.8% and 57.5%.Conclusions TGP can prevent renal tubulointerstitium injury in diabetic rats, whose mechanism may be at least partly correlated with suppression on increased expression of OPN and α-SMA.
【Fund】: 安徽省自然科学基金资助课题(No070413100);; 安徽省教育厅自然科学基金资助课题(No2006KJ316B)
【CateGory Index】: R285.5
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