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The regulation of Curcumin on the amyloidogentic pathaway of APP in Alzheimer's disease

ZHANG Hong-mei,ZHANG Xiong,LI Yu(Dept of Pathology,Chongqing Medical University,Chongqing 400016,China)  
Aim To investigate the effects of Curcumin on the APP processing in the amyloidogenic pathway in vitro and explore its mechanisms on Aβ generation inhibition.Methods Plasmids APPswe and BACE1-mychis were transiently co-transfected in SH-SY5Y cell by LipofectaminTM2000.The cell line then treated with Curcumin at 0,1.25,5,20 μmol·L-1 for 24 h,or with Curcumin at 5 μmol·L-1 for 0,12,24 and 48 h for the time course assay.RT-PCR were performed to measure the endogenous levels of APP and BACE1 mRNA.Western blot were used to detect the protein expression of BACE1 and C99,the major β-secretase cleavage product.The concentration of Aβ40/42 was detected by β-amyloid 1~40 or 42 Colorimetric ELISA.Results RT-PCR results showed that the mRNA levels of APP and BACE1 were decreased obviously in a dose-and time-dependent manner after treated with Curcumin in SH-SY5Y(P0.05).Western Blotting results showed that the expression of BACE1 and C99 protein were all decreased in a dose-and time-dependent manner(P0.05).ELISA results showed that the generation of Aβ40/42 reduced significantly,also in a dose-and time-dependent manner(P0.05).Conclusion Our finding demonstrated that Curcumin could inhibit the expression of the APP at mRNA;the expression of the BACE1 at mRNA and protein levels,furthermore it could inhibit the generation of Aβ40/42,and those changes were dose-time dependent(P0.05).Those factors are key components in APP amyloidogenic pathway.
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