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《Chinese Pharmacological Bulletin》 2009-04
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Studies on the anti-inflammatory molecular mechanism of chlorogenic acid extracted from Lonicera confusa DC in vitro

YANG Bin1,QIU Yue1,WANG Liu-ping2,ZHANG Xi-liu3(1.Dept of Pharmacology,Guangxi Medical University,Nanning 530021,China;2.Dept ofPharmacology,3.Dept of Pathology,Guangxi Traditional Chinese Medical University,Nanning 530001,China)  
Aim To investigate the anti-inflammatory molecular mechanism of chlorogenic acid extracted from Lonicera confusa DC in vitro.Methods PMΦ of a rat was segregated.MTT assay was used to detect the effects of the chlorogenic acid on PMΦ cells growth activities. PMΦ was stimulated with LPS for a prolonged period,ELISA was used to detect the level of TNF-α,IL-6 and PGE2 in the supernatant;COX-2 activity was determined by the level of PGE2 in the supernatant.After stimulating PMΦ with A23187 for a short time,the 6-keto-PGF1α level in the supernatant was measured by radioimmunoassay to express COX-1 activity.Results Chlorogenic acid had no inhibitive effects between 31.25 mg·L-1 and 1000 mg·L-1.The level of TNF-α,IL-6 and PGE2 in drug groups was lower than that of LPS-induced group,and the difference was significant,in a dose-dependent manner.The concentration of 50 mg·L-1 group was ineffective in the expression of TNF-α.Low concentration chlorogenic acid inhibited the expression of 6-keto-PGF1α,while high-concentration induced it.Conclusions The anti-inflammatory effect of chlorogenic acid may be related to inhibiting TNF-α,IL-6 activity and affecting exogenous AA metabolism.
【Fund】: 广西自然科学基金资助项目(No桂科基0575057)
【CateGory Index】: R285.5
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