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《Chinese Pharmacological Bulletin》 2010-01
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Effects of gastrin on NF-κB signaling pathway in human colon cancer cells

CAO Jun1,ZOU Xiao-ping1,YU Cheng-gong1,YU Hong-gang2 (1. Dept of Gastroenterology,Nanjing Gulou Hospital Affiliated to Medical School of Nanjing University,Nanjing 210008,China; 2. Dept of Gastroenterology,Renmin Hospital of Wuhan University,Wuhan 430060,China)  
Aim To investigate the effects of gastrin-17 on NF-κB signaling pathway in human colon cancer cells-Colo320WT. Methods All subjects were divided into four groups: control, gastrin-17,gastrin-17 + L365,260 ( gastrin-17 receptor blocker) and L365,260 group. Concerning gastrin-17 group,Colo320WT cells were treated by gastrin-17 for 12 h. Concerning gastrin-17+L360,265 group,Colo320WT cells were pretreated by L365,260 for 30 min and then were treated by gastrin-17 for 12 h. Concerning L365,260 group,Colo320WT cells were pretreated by L365,260 for 30 min. NF-κB DNA binding activity was determined by electrophoretic mobility shift assay (EMSA). Expressions of uPA protein and mRNA were assayed by Western blot and RT-PCR,respectively. Results NF-κB DNA binding activity increased more greatly in the Co-lo320WT cells stimulated by gastrin than that of the control group. And the expressions of uPA protein and mRNA were up-regulated more markedly than those of the control group. The above responses resulting from gastrin-17 were partly blocked by gastrin-17 receptor blocker,L365,260. Conclusion Gastrin-17 may activate NF-κB signaling pathway in human colonic cancer cells Colo320WT.
【Fund】: 国家自然科学基金资助项目(No30470782)
【CateGory Index】: R735.34
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