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《Chinese Pharmacological Bulletin》 2010-12
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Protective effects of Astragalus Polysaccharide on cultured myocardial cells subjected to anoxia/reoxygenation injury in neonatal rats

MIN Qing,BAI Yu-ting,YU Wei,CAI Fei,REN Ping,WU Ji-liang(The Pharmacy School of Xianning University,Xianning Hubei 437100,China)  
Aim To study the protective effects of Astragalus Polysaccharide(APS)on myocardial cells injured by anoxia/reoxygenation(A/R)in neonatal rats.Methods An anoxia/reoxygenation model of myocardial cells from neonatal Sprague Dawley(SD)rats was established.The cells were randomly divided into 5 groups:blank control group;A/R group;A/R+APS(10,30,100 mg·L-1)groups.APS was added into the culture medium with a final concentration 10,30,100 mg·L-1 before anoxia and reoxygenation.Lactate dehydrogenase(LDH),Cmatine kinase(CK)and Aspartate aminotransferase(AST)release,Malondialdehyde(MDA)contents and Superoxide dismutase(SOD)activity in the bathing medium were assayed for the evaluation of myocardial cells injury.The protective role of APS in mitochondria of myocardial cells was measured by MTT after A/R injury.Cardiomyocyte apoptosis was detected by flow eytometry.Results APS could restrain the LDH,CK and AST release evidently,decrease the MDA content and enhance the SOD activity,increase OD490 of myocardial cells injured by A/R and inhibit cardiomyocyte apoptosis.Conclusion APS can produce effect of protecting the cultured myocardial cells injured by A/R.The mechanism is related to the enhancement of antioxidative effect on cells,and the reduction membrane damage caused by free radical and lipid peroxide,and inhibit cardiomyocyte apoptosis.
【Fund】: 湖北省教育厅重点科研项目(NoD20092803)
【CateGory Index】: R285.5
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 YIN Yan-yan, LI Wei-ping, WANG Shao-bin,HE Ting, MING Liang (Dept of Pharmacology,Anhui Medical University, Hefei 230032, China);Effect of extract of astragalus on inflammatory factor and apoptosis after focal cerebral ischemia/reperfusion injury in rats[J];Chinese Pharmacological Bulletin;2005-12
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