The progress in application of embryonic stem cell-based metabonomics in toxicology research

ZHOU Hui-fang,WANG Li,ZHANG Yan-jun,BIAN Yu-hong(College of Chinese Pharmacology,Tianjin University of Tranditional Chinese Medicine,Tianjin 300193,China)  

Embryonic stem cells(ESCs)are highly undifferentiated cells with infinite ability to proliferate and to differentiate into cells from three germ layers.So,ESCs could be used as a tool for toxicity evaluation.Metabonomics is a new subject developed recently to analysis low molecular weight endogenous metabolites of an organism or cell quantitatively and qualitatively.Metabonomics is usually used for studying biological fluids,cells extract,cells culture medium and tissue by using nuclear magnetic resonance and mass spectrum techniques.In this paper we reviewed experimental techniques related to ESCs and metabonomics and its current application status in toxicology.At the same time,we approach the application and the prospect of embryonic stem cell-based metabonomics in toxicology research.

【Fund】： 中国博士后科学基金会资助项目(No200902281);; 国家重点基础研究发展计划(973计划)资助项目(No2011CB505302)
【CateGory Index】： R114

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