Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Pharmacological Bulletin》 2011-03
Add to Favorite Get Latest Update

Apoptosis of human colon adenocarcinoma cell line SW620 induced by diterpenoid C from Radix Curcumae and its related pathways

SHEN Yan1,L Bin1,ZHANG Shuo1,MA Zhong-jun2(1.Dept of Gastroenterology,the Frist Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,China;2.College of Pharmacy,ZhejiangUniversity,Hangzhou 310058,China)  
Aim To study the apoptotic effect of the new diterpenoid C from Radix Curcumae on human colon adenovarcinama SW620 cells and its underlying mechanisms.Methods The effect of diterpenoid C on cell proliferation was assessed by MTT assay,the cell apoptosis induced by diterpenoid C was determined by flow cytometry,and the expression level of ERK,p-ERK,JNK,p-JNK,p38 MAPK,p-p38 MAPK,caspase-3 was detected by Western blot analysis.Results Diterpenoid C inhibited the cell proliferation and induced apoptosis in dose-and time-dependent manners,which was significantly more potent than 5-Fluorouracil.The median inhibitory concentration(IC50) at 24,48,and 72 h were 28.31,15.58,and 6.14 mg·L-1,respectively.The expression level of ERK,JNK,p38 and their corresponding phosphorylation products was reduced by diterpenoid C in a dose-dependent manner,while the level of caspase-3 was increased.Conclusion Diterpenoid C inhibits proliferation and induces apoptosis of SW620 cells by suppressing MAPK signaling pathway and inducing apoptotic factor caspase-3.
【Fund】: 浙江省中医药普通课题研究计划(No2009CB014)
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 7 Hits
1 LIU Xiao-zhen1,ZHANG Hong-ying1,2(1.Department of Morphology,Dalian Medical University,Dalian 116044,China;2.National Chromatographic Research and Analysis Center,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China);Molecular mechanism of effect of curcumin on tumor[J];Journal of Dalian Medical University;2008-05
2 YU Linfeng, LV Bin, XU Lei, CHEN Mingtao, NI Guibao. Department of Gastroenterology, First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou (310006);Effects of Curcumae on Expressions of Vascular Endothelial Growth Factor and Cyclooxygenase-2 in Gastric Mucosa of Rats Fed with MNNG[J];Chinese Journal of Gastroenterology;2007-03
3 WEN Jun, XU Ming (Laboratory of Molecular Pharmacology, Centralsouth University, Changsha 410078);Inhibition of p38 MAPK kinase enhances diallyl disulfide-induced apoptosis in human CNE2 cells[J];Chinese Pharmacological Bulletin;2003-04
4 LIANG Lei1,ZHANG Xu-hui3,WANG Xiao-yan2,CHEN Yan1,DENG Hong-zhu1(1.School of TCM;2.Dept of Pathology,Southern Medical University,Guangzhou 510515,China;3.Dept of Oncology,the Second People's Hospital of Guangdong Province,Guangzhou 510317,China);Effect of sophoridine on proliferation and apoptosis of human colon adenocarcinoma cells(SW620)[J];Chinese Pharmacological Bulletin;2008-06
5 XU Yi, LU Bin, XIANG Baikang,et al Department of Gastroenterology, Traditional Chinese Medicine Hospital o f Zhejiang Province, Hangzhou 310006;Effect of Extract of Radix Curcumae on Somatostatin in Plasm and Gastric Tissue[J];Chinese Journal of Integrated Traditional and Western Medicine On Gastro-spleen;2004-04
6 FANG Lu-min,HANG Zhen(College of Pharmaceutical Sciences,Zhejiang Chinese Medical Univesity,Huangzhou 310053,Zhejiang,China);Research Progress of Radix Curcumae[J];Chinese Archives of Traditional Chinese Medicine;2008-09
7 ZHOU Si-gui1,YUAN Xi3,PAN Xue-diao1,JIN Gui-fang1,XU Li-peng2(1.Dept of Pharmacology,GuangDong Pharmaceutical University,Guangzhou 510006,China;2.Institute of New Drug Research,Jinan University College of Pharmacy,Guangzhou 510632,China;3.Dept of Clinical Medicine,Zhongshan School of Medicine,Sun Yat-sen University,GuangZhou 510080,China);ERK 1/2 and p38 MAPK mediate the effect of BAPTA-AM inhibiting RANKL-induced bone marrow macrophages differentiation[J];Chinese Pharmacological Bulletin;2010-09
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 JIN Hai-feng1,LV Bin1,CHEN Zhe1,MA Zhong-jun2(1He Frist Affi liated Hospital,Zhejiang Chinese Medical University,Hangzhou 310006,China;2College of Pharmacy,Zhejiang University,Hangzhou 310058,China);Effect of diterpenoid C from Radix Curcumae on proliferation of human gastric cancer cells SGC-7901[J];China Journal of Traditional Chinese Medicine and Pharmacy;2010-05
2 WANG Qi-rui1, LI Chun-hua1, FU Xiu-qiong1, LIU Ya-wei2, TANG Jing3, FAN Qin1, SUN Xue-gang1 1Laboratory of Molecular Biology, School of Traditional Chinese Medicine, 2School of Basic Medical Science, 3Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Effects of sophoridine on the growth and expressions of p53 and vascular endothelial growth factor of transplanted solid tumor SW480 in nude mice[J];Journal of Southern Medical University;2010-07
3 YI Xin-Ping MA Jun-Gang(Institute of Supervision & Inspection on Product Quality,Urumqi 830017,P.R.China);Study on Chemical Components of Volatile Oil in the Root of Curcuma Wenyujin in Xinjiang[J];Chinese Journal of Spectroscopy Laboratory;2010-05
4 YANG Ke,TANG Hai-lin,SU Qi,et al (Medical College of Nanhua University,Hengyang,Hunan 421001,China);Apoptosis of Nasopharyngeal Carcinoma Cells Line CNE1 Induced by Diallyl Disulfide[J];Journal of Nanhua University(Medical Edition);2008-03
5 ZHANG Shu-fang, SUN Ji-feng, ZHANG Li, SONG Ying-ming(Department of Biochemistry, Basic Medical School, Changchun Medicial College, Changchun 130031, China);Inhibition of Growth and Induction of Apoptosis of Human Laryngeal Cancer Hep-2 Cells by Curcumin[J];Chinese Journal of Biologicals;2010-10
6 Lei Xu Bin Lv Lin-Feng Yu,Department of Gastroenterology,the First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine,Hangzhou 310006,Zhejiang Province,China;Chemoprevention of gastric cancer in rats by a wetdistillation extract of radix curcumae[J];World Chinese Journal of Digestology;2007-24
7 LING Hui, SONG Ying,ZENG Tie-Bing, YUAN Jing-Ping, SU Qi (Cancer Research Institute,Nanhua University,Hengyang 421001);Role of ERK/AP-1 pathway in the differentiation induced by DADS on human gastric caner cell[J];Chinese Pharmacological Bulletin;2003-11
8 ZHU Hui-Ming, WU Tie, CUI Liao (Dept of Pharmacology,Guangdong Key Laboratory for Research and Development of Natural Drugs Guangdong Medical College,Zhanjiang 524023);Study of aspirin on proliferation and apoptosis of human lung Adenocarcinoma cell lines SPCA-1 in vivo[J];Chinese Pharmacological Bulletin;2004-06
9 WANG Li-Hui,DUAN Hui-Jun, SHI Yong-Hong,LIU Qing-Juan,HE Ning,LIU Shu-Xia,GAO Feng (Dept of Pathology,Hebei Medical University,Shijiazhuang 050017,China);Effects of losartan on renal function and expression of phospho-p38 mitogen-activated protein kinase in early diabetic glomeruli[J];Chinese Pharmacological Bulletin;2004-12
10 LUO Guo-gang1,MA Ai-qun1,XU Cang-bao~2,3 ,CAO Yong-xiao2,Lars EDVINSSON~2,3 (1.The First Hospital Medical School of Xi'an Jiaotong University,2.Dept of Pharmary of Xi'an Jiaotong University, Xi'an 710061,China; 3.Division of Experimental Vascular Research, Institution of Medicine, Lund University, Sweden,S-221 84);Involvement of ERK1/2 pathway in endothelin ET_A and ET_B receptor-mediated contraction in isolated rat mesenteric artery[J];Chinese Pharmacological Bulletin;2005-03
China Proceedings of conference Full-text Database 1 Hits
1 HU Peng-yi,ZHENG Qing,YUE Peng-fei,WU Zhen-feng,QIAN Jia,YANG Ming* (KeyLaboratoryofModernPreparationofTCM(Jiangxi University of Traditional Chinese Medicine),Ministry of Education,Nanchang 330004,Jiangxi,China);Advance in the Pharmacological Research on Sophoridine in the Last Decade[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 CUI Cheng, WANG Run-Tian, TONG Hui, WANG Zhi-Hua, DENG Yu-Qing Department of Immunology, Institute of Basic Medicine, Hebei Medical University,Shijiazhuang 050017,China;Study in vitro on down-regulating effect of Matrine N-Oxide on immunosuppression of murine Colon26 tumor cells[J];Journal of the Fourth Military Medical University;2006-21
2 ZHANG Cui-mei , FENG Zhi-chun Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China;Matrine-induced gamma-globin synthesis in K562 cells[J];Journal of Southern Medical University;2007-02
3 Liu Xiaojing Zhang Zheng Yang Li Qiang OuInternal Medicine Department, The First Affiliated Hospital (Chengdu 610041);Regulative Effects Pentagastrin and Somatostatin on Growthof Human Gastric Cancer Cells in Vitro[J];JOURNAL OF WEST CHINA UNIVERSITY OF MEDICAL SCIENCES;1998-03
4 Wang Luping, 2Yu Jiyao, 1Ding Huaye, et al(1General Hospital of Beijing Command,Beijing 100700;2General Hospital of Navy,Beijing 100037);Sognificance of prognosis and DNA content of somatostatin in gastric carcinoma[J];CHINESE JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOG;1998-01
5 Zou Zhongmei (Institute of Medicinal Plants, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100094);Historical Evolution of Methods for Preparation of Radix Curcumac (Yu Jin) and Their Research Progress[J];;2003-05
6 Zhang Qingyun Zhang Guorong Yin Chang'an Du Yanping Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002) Ningxia Agricultural College, Yinchuan 750002) Yan Chi Factory of Zi Jinhua Pharmaceutical Group, Yinchuan 750002);Protection and Utilization of Sophora Alopecuroides L. as Medicinal Plants Resource[J];;2006-01
7 HU Run-huai,SHAO Qing-song(Zhejiang Forestry University,Hangzhou,311300,China);Research Advances on Chemical Constituents of Curcuma wenyujin[J];Lishizhen Medicine and Materia Medica Research;2007-07
8 CAO Dong-xu~(1,2),YUAN Ying-jin~1 (1.College of Food Engineering and Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China;2.School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;);Effect of Sophocarpine on Inhibiting Proliferation and Inducing Apoptosis in Human Erythroleukemia K562 Cells[J];Journal of Tianjin University of Science & Technology;2006-04
9 Zhang Hao, Hsieh Chenko (School of Pharmacy,West China University of Medical Sciences,Chengdu 610041)Jiao Wenxn (Department of Pharmacy,Xian University of Medical Sciences,Xian 710061);STUDIES ON ESSENTIAL OIL FROM CHINESE TRADETIONAL DRUG YUJIN (TUBERS OF CURCUMA)[J];Natural Product Research and Development;1997-01
10 Zhang Hao,Xie Chengke (School of Pharmacy,West China University of Medical Sciences, Chengdu 610041)Jiao Wenxu (School of Pharmacy,Xian University of Medical Sciences, Xian 710061);DETERMINATION OF CURCUMINOIDS AND INORGANIC ELEMENTS IN CHINESE TRADITIONAL DRUG YUJIN(TUBERS OF CURCUMA)[J];Natural Product Research and Development;1997-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved