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Apoptosis of human colon adenocarcinoma cell line SW620 induced by diterpenoid C from Radix Curcumae and its related pathways

SHEN Yan1,L Bin1,ZHANG Shuo1,MA Zhong-jun2(1.Dept of Gastroenterology,the Frist Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,China;2.College of Pharmacy,ZhejiangUniversity,Hangzhou 310058,China)  
Aim To study the apoptotic effect of the new diterpenoid C from Radix Curcumae on human colon adenovarcinama SW620 cells and its underlying mechanisms.Methods The effect of diterpenoid C on cell proliferation was assessed by MTT assay,the cell apoptosis induced by diterpenoid C was determined by flow cytometry,and the expression level of ERK,p-ERK,JNK,p-JNK,p38 MAPK,p-p38 MAPK,caspase-3 was detected by Western blot analysis.Results Diterpenoid C inhibited the cell proliferation and induced apoptosis in dose-and time-dependent manners,which was significantly more potent than 5-Fluorouracil.The median inhibitory concentration(IC50) at 24,48,and 72 h were 28.31,15.58,and 6.14 mg·L-1,respectively.The expression level of ERK,JNK,p38 and their corresponding phosphorylation products was reduced by diterpenoid C in a dose-dependent manner,while the level of caspase-3 was increased.Conclusion Diterpenoid C inhibits proliferation and induces apoptosis of SW620 cells by suppressing MAPK signaling pathway and inducing apoptotic factor caspase-3.
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