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The hypoglycemic effect of Laminaria japonica on diabetic model induced by alloxan in rats

YU Zhu-qin1,LI Xiao-dan1,XU Xin-ying1,GUO Yun-liang1,SHUAI Li2,DUAN De-lin3,FU Peng-fei4(1.Institute of Cerebrovascular Diseases,Qingdao University Medical College,Qingdao 266003,China;2.School of Chemistry,Chemical Engineering and Environmental Sciences,Qingdao University,Qingdao 266071,China;3.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;4.Shandong Haizhibao Marine Sci.& Tech,Ltd Co Rongcheng 264300,China)  
Aim To investigate the hypoglycemic effects and its mechanism of Laminaria japonica(L.japonica) noodle in diabetic model rats induced by alloxan.Methods Sixty healthy male rats were used to establish diabetic models by injecting peritoneally alloxan,and the L.japonica noodle,consisting of low(2.5 g·kg-1),middle(10 g·kg-1) and high(25 g·kg-1) dosage treated groups,and was applied as additive in forage to treat the rats.The levels of fasting blood glucose(FBG) were detected by automatic blood glucose device.Enzyme linked immunosorbent assay(ELISA) was applied to determine the insulin level in serum.The serum levels of lipid perioxide malondialdehyde(MDA) were measured by thiobarbituric acid assay and nitric oxide(NO) by nitrate reductase assay.The activities of superoxide dismutase(SOD) were determined by xanthinoxidase assay and glutathione peroxidase(GSH-Px) by chemical colormetry.Results After treated with kelp(L.japonica) noodle powder,the serum levels of insulin increased significantly than those in the model group,while the FBG in the middle-high dosage treated groups decreased significantly than that in the model group(P0.05).The levels of MDA and NO in kelp(L.japonica) noodle treated group were lower than those in the model group,while the activities of SOD and GSH-Px were higher than those in the model group,of which the significant difference existed between the middle-high dosage treated groups and the model group(P0.05).There were no significant differences between the middle and high dosage treated groups in above indexes(P0.05).Conclusion L.japonica noodle might enhance islet cell secreting function to recovery to reduce the level of FBG by antioxidant effect,and its ideal treated dosage is 10 g·kg-1 body weight each day.
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