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《Chinese Pharmacological Bulletin》 2012-11
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Effects of saponin of pearsonothuria graeffei on bone marrow cells in myelosuppression mice induced by cyclophosphamide

HE Min,WANG Jing-feng,LIU Dong,LI Bing,WANG Yu-ming,XUE Chang-hu(College of Food Science and Engineering,Ocean University of China,Qingdao Shandong 266003,China)  
Aim To investigate the effects of saponin of pearsonothuria graeffei(SP)on cell cycle and apoptosis of bone marrow cells in cyclophosphamide(CY)-induced myelosuppression mice.Methods The myelosuppression mice model was generated after injected intraperitoneally with CY(100 mg·kg-1).The myelosuppression mice were divided into three groups: the model control group and saponin group treated with SP 10mg/(kg·d) respectively.The other mice were given with 0.5% CMC solution as placebo.All agents were administered once a day for 16 d.Changes in cell cycle and apoptosis of bone marow cells were detected with FCM(flow cytometry).The expressions of cyclinB1,bcl-2,bcl-xl were determined with reverse transcription PCR(RT-PCR).Results Saponin of pear-sonothuria graeffei significantly elevated bone marrow nucleated cell(BMNC) and promoted the cell cycle from S phase to G 2 / M phase,the expression of cyclinB1 was also increased in saponin group.The apoptosis rate in myelosuppression mice was significantly reduced and the expression levels of bcl-2 and bcl-xl were elevated in saponin group compared with the model control mice.Conclusion Saponin of pearsonothuria graeffei is beneficial for improving bone marrow cells division and proliferation,inhibiting cell apoptosis,recovering the function of hematopoiesis.
【Fund】: 国家自然科学基金资助项目海洋公益性行业科研专项(No 201105029 31000795);; “泰山学者”建设工程专项经费
【CateGory Index】: R285.5
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